HPLC-UV analysis of phenolic compounds and biological activities of Padina pavonica and Zanardinia typus marine macroalgae species

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.55730/1300-008x.2761

Hatice Banu Keskinkaya, Ebru Deveci, Bahar Yilmaz Altinok, Numan Emre Gümüş, E. Aslan, Cengiz Akköz, S. Karakurt

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Abstract

tr Abstract: The marine macroalgae species are recognized as the food of the future with excellent bioactive properties. This study aimed to investigate phenolic compounds; cytotoxic, antibacterial, and antioxidant activities; total phenolic (TPC) and flavonoid (TFC) contents of the methanol, ethanol, and water extracts of Padina pavonica (PP) and Zanardinia typus (ZT) . To the best of our knowledge, this is the first report in which ZT was evaluated in terms of phenolic content, antioxidant, antibacterial, and cytotoxic activities. The HPLC analysis allowed the identification of five phenolic compounds containing rutin (0.31 ± 0.04 – 3.00 ± 0.21 ppm) in all extracts and trans - p -coumaric acid (0.15 ± 0.02 – 3.95 ± 0.02 ppm) in MPP, EPP, MZT, EZT, WZT as the major compounds. TPC and TFC of the extracts were calculated as 11.78 ± 0.75 – 76.78 ± 0.54 µg GAEs/mg extract and 6.78 ± 0.17 – 29.50 ± 2.23 µg QEs/mg extract, respectively. The highest cytotoxicity was observed in EZT (CC 50 : 132.3 ± 22.4 µg/mL) against MCF-7 and MZT (CC 50 : 91.4 ± 20.9 µg/mL) against MIA PaCa-2. Among the studied extracts, EPP showed the best antibacterial activity against all test pathogens. Also, EPP indicated superior antibacterial activity against Plesiomonas shigelloides (MIC: 1.25 mg/mL) and Staphylococcus aureus (MIC: 1.25 mg/mL). EZT displayed the highest antioxidant activity in DPPH • (IC 50 : 49.03 ± 0.28 µg/mL), CUPRAC (A 0.50 : 15.20 ± 0.14 µg/mL), and ABTS •+ (IC 50 : 18.86 ± 0.74 µg/mL) assays. The results approved that P. pavonica and Z. typus marine macroalgae species could be valued as natural sources of bioactive agents for food and pharmacology applications.

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帕迪纳和扎纳迪纳斯海洋大型藻类酚类化合物及生物活性的HPLC-UV分析

tr摘要：海洋大型藻类以其优异的生物活性被认为是未来的食物。本研究旨在研究酚类化合物；细胞毒性、抗菌和抗氧化活性；木瓜（PP）和番荔枝（ZT）的甲醇、乙醇和水提取物的总酚（TPC）和黄酮（TFC）含量。据我们所知，这是第一份对ZT进行酚类含量、抗氧化、抗菌和细胞毒性评估的报告。高效液相色谱分析可以鉴定出所有提取物中含有芦丁（0.31±0.04–3.00±0.21 ppm）和MPP、EPP、MZT、EZT和WZT中反式-对-香豆酸（0.15±0.02–3.95±0.02 ppm）的五种酚类化合物为主要化合物。提取物的TPC和TFC分别计算为11.78±0.75–76.78±0.54µg GAEs/mg提取物和6.78±0.17–29.50±2.23µg QEs/mg提取物。EZT（CC 50:132.3±22.4µg/mL）对MCF-7和MZT（CC 50:91.4±20.9µg/mL。在所研究的提取物中，EPP对所有测试病原体显示出最佳的抗菌活性。此外，EPP对志贺邻单胞菌（MIC:1.25mg/mL）和金黄色葡萄球菌（MIC:11.25mg/mL）显示出优异的抗菌活性。EZT在DPPH•（IC 50:49.03±0.28µg/mL）、CURAC（A 0.50:15.20±0.14µg/mL.）和ABTS•+（IC 50:18.86±0.74µg/mL。结果表明，P.pavonica和Z.typus海洋大型藻类可作为食品和药理学应用的生物活性剂的天然来源。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.