Effect of NADPH on myocardial ischemia-reperfusion injury in rats

Q4 Medicine

中华麻醉学杂志 Pub Date : 2019-10-20 DOI:10.3760/CMA.J.ISSN.0254-1416.2019.10.010

Yi-fei Wang, Jiang Zhu, Pei-min Chen, Liuhui Chang

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Abstract

Objective To evaluate the effect of nicotinamide adenine dinucleotide phosphate (NADPH) on myocardial ischemia-reperfusion (I/R) injury in rats. Methods Fifty-six SPF adult male Sprague-Dawley rats, weighing 220-320 g, aged 1-2 months, were divided into 4 groups (n=14 each) using a random number table method: sham operation group (Sham group), myocardial I/R group (I/R group), NADPH group (N group) and diltiazem group (D group). The model of myocardial I/R injury was established by ligation of the left anterior descending branch for 30 min followed by 2-h reperfusion in anesthetized rats.NADPH 16 mg/kg was intravenously infused over 5 min starting from 5 min of reperfusion in N group.Diltiazem 5 mg/kg was infused through the internal jugular vein starting from 10 min before ischemia until the end of ischemia.At 2 h of reperfusion, blood samples were taken from the internal jugular vein for measurement of serum LDH and cTnI concentrations, and myocardial tissues were taken for determination of infarct size and ROS level. Results Compared with Sham group, the serum LDH and cTnI concentrations, myocardial infarction size and ROS levels were significantly increased in I/R group (P 0.05). Conclusion NADPH can reduce myocardial I/R injury through antioxidant effect in rats. Key words: NADPH; Myocardial reperfusion injury

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NADPH对大鼠心肌缺血再灌注损伤的影响

目的探讨烟酰胺腺嘌呤二核苷酸磷酸(NADPH)对大鼠心肌缺血再灌注(I/R)损伤的影响。方法选用SPF级成年雄性sd - dawley大鼠56只，体重220 ~ 320 g, 1 ~ 2月龄，采用随机数字表法分为4组(n=14):假手术组(sham组)、心肌I/R组(I/R组)、NADPH组(n组)和地尔硫平组(D组)。麻醉大鼠左前降支结扎30min，再灌注2h，建立心肌I/R损伤模型。N组从再灌注5 min开始，5 min内静脉滴注NADPH 16 mg/kg。地尔硫卓5 mg/kg于缺血前10 min经颈内静脉滴注至缺血结束。再灌注2 h取颈内静脉血，测定血清LDH和cTnI浓度，取心肌组织测定梗死面积和ROS水平。结果与Sham组比较，I/R组大鼠血清LDH、cTnI浓度、心肌梗死面积、ROS水平均显著升高(P < 0.05)。结论NADPH可通过抗氧化作用减轻大鼠心肌I/R损伤。关键词:NADPH;心肌再灌注损伤

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