Changes in salicylic acid content and pathogenesis - related (PR2) gene expression during barley - Pyrenophora teres interaction

Q3 Agricultural and Biological Sciences

Hellenic Plant Protection Journal Pub Date : 2018-07-01 DOI:10.2478/hppj-2018-0010

A. Al-Daoude, M. Jawhar, E. Al-shehadah, A. Shoaib, M. Orfi, M. Arabi

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引用次数: 5

Abstract

Abstract Net blotch (NB), caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres, substantially reduces barley grain yield and quality worldwide. The role of salicylic acid (SA) signaling in NB resistance has been poorly documented. In this study, SA levels as well as the expression of the SA-responsive gene PR2 were monitored in infected leaves of two barley genotypes, Banteng (resistant) and WI2291 (susceptible), at different time points of infection. SA signaling was activated in bothgenotypes 24 hours post infection (hpi) as compared with non-inoculated plants. However, with or without pathogen pretreatment, SA signifi cantly increased (P=0.001) in Banteng comparing with WI2291. RT-PCR analysis revealed that PR2 expression increases in the resistant and susceptible genotypes over the inoculation time points, with maximum expression (6.4 and 1.99-fold, respectively) observed 6 dpi. PR2 expression was paralleled by an increase in leaf SA content as shown by the test coincidence (F3, 32 = 4.74, P = 0.001). Based on barley genotype resistance levels, our data strengthen the idea that SA signaling and PR2 play a role in barley NB reduction

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大麦-嗜黄菌相互作用中水杨酸含量及发病机制相关基因(PR2)表达的变化

摘要由坏死性真菌病原体Pyrenophora teres f.teres引起的净斑点病（NB）在全球范围内严重降低了大麦的产量和品质。水杨酸（SA）信号传导在NB抗性中的作用一直没有得到充分的证明。在本研究中，在不同的感染时间点，监测了两种大麦基因型，斑藤（抗性）和WI2291（易感）的SA水平以及SA响应基因PR2的表达。与未接种的植物相比，SA信号在两种基因型感染后24小时（hpi）都被激活。然而，与WI2291相比，在病原体预处理或不预处理的情况下，班腾的SA显著增加（P=0.001）。RT-PCR分析显示，随着接种时间的推移，PR2在抗性和易感基因型中的表达增加，在6dpi时观察到最大表达（分别为6.4和1.99倍）。PR2的表达与叶片SA含量的增加平行，如测试一致性所示（F3，32=4.74，P=0.001）。基于大麦基因型抗性水平，我们的数据强化了SA信号传导和PR2在大麦NB减少中起作用的观点

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hellenic Plant Protection Journal Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.50

自引率

0.00%

发文量