Thin-layer hydration method to prepare a green tea extract niosomal gel and its antioxidant performance

European Pharmaceutical Journal Pub Date : 2021-01-01 DOI:10.2478/afpuc-2021-0011

U. Chasanah, N. Mahmintari, F. Hidayah, F.A. El Maghfiroh, D. Rahmasari, R. Weka Nugraheni

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引用次数: 1

Abstract

Abstract This study aimed to prepare a niosomal gel of green tea (Camellia sinensis) extract containing catechins, mostly epigallocatechin-3-gallate (ECGC), as a potent antioxidant. Niosomes can increase EGCG's stability and penetration into the skin for a better therapeutic effect. Niosomes were prepared by a thin-layer hydration method, were evaluated for their vesicle shape, particle size, polydispersity index, zeta potential and entrapment efficiency, and then incorporated into gels using sodium alginate as a gelling agent. Three niosomal gel formulations were prepared with different concentrations of niosomes green tea extract. Afterwards, organoleptic properties, chemical and physical characteristics, antioxidant activity, and stability and irritability of the niosomal gels were investigated. The different concentrations of green tea extract had a significant effect on the physical characteristics, but not on the chemical ones. Its antioxidant activity was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging method. The 50% extract green tea niosomes gel showed the highest inhibition value (25.13%). The stability was determined by freeze–thaw and real-time methods; they showed a decrease in pH, but still within the pH range of skin. The irritability test used was the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) method, which showed no irritation for all formulas. In conclusion, 50% green tea extract niosomes gel results showed it to be the best formulation with optimal antioxidant results.

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薄层水化法制备绿茶提取物乳糖凝胶及其抗氧化性能

摘要本研究旨在制备一种含有儿茶素（主要是表没食子儿茶素-3-没食子酸酯（ECGC））的绿茶（山茶）提取物的niosomal凝胶，作为一种有效的抗氧化剂。Niosomes可以提高EGCG的稳定性并渗透到皮肤中，从而获得更好的治疗效果。通过薄层水合法制备了Niosomes，并对其囊泡形状、粒径、多分散指数、ζ电位和包封效率进行了评估，然后使用海藻酸钠作为胶凝剂将其掺入凝胶中。用不同浓度的niosomes绿茶提取物制备了三种niosomes凝胶制剂。然后，研究了niosol凝胶的感官特性、化学和物理特性、抗氧化活性以及稳定性和易激性。不同浓度的绿茶提取物对其物理特性有显著影响，但对其化学特性没有影响。采用1，1-二苯基-2-苦基肼（DPPH）清除法测定其抗氧化活性。50%提取物的绿茶niosomes凝胶显示出最高的抑制值（25.13%）。通过冻融和实时方法测定其稳定性；它们显示出pH降低但仍在皮肤的pH范围内。使用的过敏性测试是母鸡蛋测试绒毛尿囊素膜（HET-CAM）方法，该方法对所有配方奶粉均无刺激性。综上所述，50%的绿茶提取物niosomes凝胶结果表明，它是具有最佳抗氧化效果的最佳配方。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Pharmaceutical Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： European Pharmaceutical Journal publishes only original articles not previously published and articles that are not being considered or have not been submitted for publication elsewhere. If parts of the results have been published as conference abstract or elsewhere, it should be stated in references. The ethical standards of the Helsinki-Tokio Declaration should be kept. This should be mentioned in the Methods of manuscript. Reviews are published only on request. Authors, whose submitted research work was performed with the support of a company, should indicate this in Conflict of Interest.