Histochemical and surface microstructural analyses of floral cuticles provide evidence for differential behaviors in scent volatiles emission in two tuberoses (Agave amica (Medik.) Theide and Govaerts) cultivars from Asparagaceae

IF 1.5 4区生物学 Q3 PLANT SCIENCES

Turkish Journal of Botany Pub Date : 2023-01-01 DOI:10.55730/1300-008x.2758

R. Bhattacharya, A. Mitra

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引用次数: 0

Abstract

: Two popular cultivars of tuberose ( Agave amica (Medik.) Theide and Govaerts syn. Polianthes tuberosa L.) from the family Asparagaceae, widely cultivated for floricultural purposes are ‘Calcutta Single’ (CS) and ‘Calcutta Double’ (CD). It was evidenced that CS flowers emit intense scent volatiles as compared to CD flowers, while CD flowers retained higher contents of internal pool of scent volatiles than CS flowers. Such contrasting observations between the flowers of two cultivars suggest the role of possible barriers such as cuticles that prevent the emission of scent volatiles. The cuticular structure of both cultivars was studied using different histochemical approaches, including light and epi-fluorescence microscopies. Scanning electron microscopy (SEM) was used to understand the cuticular deposition in the cultivars. Our observations confirmed relatively higher cuticular thickness in CD flowers than in CS. SEM results showed the accumulation of wax crystalloids in CS flower and the formation of cuticular nanoridges in CD flowers over the surfaces of the tepals. In addition, accumulation of lipid droplets was also noticed on the tepal peels of the cultivars showing a denser deposition in CD. Our observations suggest that the presence of wax crystalloids in CS flowers enhances the emission of volatiles, while in CD flowers, the presence of thick cuticular nanoridges possibly deters the scent emission and accumulated as an internal pool in higher amounts. The stomatal behavior also indicates a proportional relationship between cuticular deposition and scent emission; low cuticular deposition corresponds to higher emission of floral volatiles emission and vice versa as observed in CS and CD flowers, respectively.

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花角质层的组织化学和表面微观结构分析为芦笋科两个块茎（Agave amica（Medik.）Theide和Govaerts）品种香味挥发物释放的差异行为提供了证据

：两个流行的晚香玉品种（龙舌兰（Medik.）Theide和Govaerts syn。Polianthes tuberosa L.）是天门冬科的一种，广泛用于花卉栽培目的，有“加尔各答单花”（CS）和“加尔各答双花”（CD）。结果表明，与CD花相比，CS花释放出强烈的气味挥发物，而CD花比CS花保留了更高的内部气味挥发物库含量。两个品种的花朵之间的这种对比观察表明，角质层等可能的屏障可以阻止气味挥发物的释放。采用不同的组织化学方法，包括光镜和荧光显微镜，对两个品种的表皮结构进行了研究。利用扫描电子显微镜（SEM）了解了品种表皮的沉积情况。我们的观察证实CD花的表皮厚度相对高于CS花。扫描电镜结果显示，蜡晶在CS花中积累，并在花被表面形成CD花中的表皮纳米脊。此外，在CD中沉积更密集的品种的花被皮上也注意到脂滴的积累。我们的观察结果表明，CS花中蜡晶体的存在增强了挥发物的释放，而在CD花中，厚表皮纳米脊的存在可能会阻止气味的释放，并以更高的量作为内部池积累。气孔行为也表明角质沉积和气味释放之间存在比例关系；在CS和CD花中分别观察到，低表皮沉积对应于较高的花挥发物排放，反之亦然。

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来源期刊

Turkish Journal of Botany PLANT SCIENCES-

CiteScore

2.90

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The Turkish Journal of Botany is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts manuscripts (in English) covering all areas of plant biology (including genetics, evolution, systematics, structure, function, development, diversity, conservation biology, biogeography, paleobotany, ontogeny, functional morphology, ecology, reproductive biology, and pollination biology), all levels of organisation (molecular to ecosystem), and all plant groups and allied organisms (algae, fungi, and lichens). Authors are required to frame their research questions and discuss their results in terms of major questions in plant biology. In general, papers that are too narrowly focused, purely descriptive, or broad surveys, or that contain only preliminary data or natural history, will not be considered (*). The following types of article will be considered: 1. Research articles: Original research in various fields of botany will be evaluated as research articles. 2. Research notes: These include articles such as preliminary notes on a study or manuscripts on the morphological, anatomical, cytological, physiological, biochemical, and other properties of plant, algae, lichen and fungi species. 3. Reviews: Reviews of recent developments, improvements, discoveries, and ideas in various fields of botany. 4. Letters to the editor: These include opinions, comments relating to the publishing policy of the Turkish Journal of Botany, news, and suggestions. Letters should not exceed one journal page. (*) 1. Raw floristic lists (of algae, lichens, fungi, or plants), species descriptions, chorological studies, and plant sociology studies without any additional independent approaches. 2. Comparative morphology and anatomy studies (that do not cover a family, tribe, subtribe, genus, subgenus, section, subsection, or species complexes with taxonomical problems) without one or more independent additional approaches such as phylogenetical, micromorphological, chromosomal and anatomical analyses. 3. Revisions of family, tribe, genus, subgenus, section, subsection, or species complexes without any original outputs such as taxonomical status changes, IUCN categories, and phenological and ecological analyses. 4. New taxa of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group. New records of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 3 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms. 5. New taxa of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group. New records of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 5 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms.