New validated Eucalyptus SSR markers located in candidate genes involved in growth and plant development

IF 0.8 4区农林科学 Q3 FORESTRY

Forest Systems Pub Date : 2021-02-03 DOI:10.5424/fs/2020293-17074

Cintia V. Acuña, J. Rivas, N. Aguirre, Pamela Villalba, María C Martínez, M. Garcia, H. Hopp, S. Marcucci-Poltri

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引用次数: 0

Abstract

Aim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species.Area of study: Eucalyptus species from different Australian origins planted in Argentina.Materials and methods: In total, 134 SSR in 129 candidate genes (CG-SSR) involved in plant development were selected and physically mapped to the E. grandis reference genome by bioinformatic tools. Experimental validation and polymorphism analysis were performed on 48 individuals from E. grandis and interspecific hybrids (E. grandis x E. camaldulensis; E. grandis x E. tereticornis), E. globulus, E. maidenii, E. dunnii and E. benthamii.Main results: 131 out of 134 CG-SSR were mapped on the 11 chromosomes of E. grandis reference genome. Most of the 134 analyzed SSR (> 75%) were positively amplified and 39 were polymorphic in at least one species. A search of annotated genes within a 25 kbp up and downstream region of each SSR location retrieved 773 genes of interest.Research highlights: The new validated and characterized CG-SSR are potentially suitable for comparative QTL mapping, molecular marker-assisted breeding (MAB) and population genetic studies across different species within Symphyomyrtus subgenus.Keywords: CG-SSR; cross-transferability; EST; eucalypts; microsatellite.

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新鉴定的桉树生长发育候选基因SSR标记

研究目的:验证和鉴定桉树中与生长和植物发育性状相关的基因组转录序列中的微卫星或SSR标记。研究范围:在阿根廷种植的来自不同澳大利亚起源的桉树物种。材料与方法:利用生物信息学工具，从129个候选植物发育基因(CG-SSR)中筛选出134个SSR，并将其物理定位到大黄参比基因组中。对48个大叶豆和种间杂交品种(大叶豆x camaldulensis;大叶蝉(E. grandis x E. tereticornis)、globulus、E. maidenii、E. dunnii和E. benthamii。主要结果:134个CG-SSR分子标记中有131个被定位在大叶黄参考基因组的11条染色体上。134个SSR中大部分(约75%)被阳性扩增，39个SSR至少在一个物种中存在多态性。在每个SSR位点的上下25 kbp区域内搜索带注释的基因，检索到773个感兴趣的基因。研究重点:新鉴定的CG-SSR可用于合胞菌亚属不同种间的比较QTL定位、分子标记辅助育种(MAB)和群体遗传研究。关键词:CG-SSR;cross-transferability;美国东部时间;桉树林里。桉树;微卫星。

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来源期刊

Forest Systems FORESTRY-

CiteScore

1.40

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Forest Systems is an international peer-reviewed journal. The main aim of Forest Systems is to integrate multidisciplinary research with forest management in complex systems with different social and ecological background