Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances on Pseudomonas aeruginosa Isolated from Chronic Wounds

Q3 Biochemistry, Genetics and Molecular Biology

Avicenna journal of medical biotechnology Pub Date : 2022-09-03 DOI:10.18502/ajmb.v14i4.10487

Akram Etemadinia, A. Seyfoori, A. Rahimi Foroushani, R. Mazaheri Nezhad Fard, R. Bakhtiari

{"title":"Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances on Pseudomonas aeruginosa Isolated from Chronic Wounds","authors":"Akram Etemadinia, A. Seyfoori, A. Rahimi Foroushani, R. Mazaheri Nezhad Fard, R. Bakhtiari","doi":"10.18502/ajmb.v14i4.10487","DOIUrl":null,"url":null,"abstract":"Background: Biofilm formation helps Pseudomonas aeruginosa (P. aeruginosa) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropylacrylamide microgels (PNIPAM) on P. aeruginosa. Methods: Totally, 100 P. aeruginosa strains were isolated from chronic wound infections. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, algD, lasR, and PA2714 genes were amplified to investigate gene frequencies and expression rates. Results: Significant decreases were seen in lasR expression in EDTA-treated samples. Significant decreases were observed in expression of algD and lasR treated with xylitol. Decreased expression of PA2714 was seen in samples treated with xylitol with no significance. Conclusion: The PNIPAM containing xylitol or EDTA could penetrate biofilms of P. aeruginosa and significantly decrease expression of lasR and algD. This can be a novel strategy in the management of chronic wounds.","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avicenna journal of medical biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ajmb.v14i4.10487","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Biofilm formation helps Pseudomonas aeruginosa (P. aeruginosa) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropylacrylamide microgels (PNIPAM) on P. aeruginosa. Methods: Totally, 100 P. aeruginosa strains were isolated from chronic wound infections. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, algD, lasR, and PA2714 genes were amplified to investigate gene frequencies and expression rates. Results: Significant decreases were seen in lasR expression in EDTA-treated samples. Significant decreases were observed in expression of algD and lasR treated with xylitol. Decreased expression of PA2714 was seen in samples treated with xylitol with no significance. Conclusion: The PNIPAM containing xylitol or EDTA could penetrate biofilms of P. aeruginosa and significantly decrease expression of lasR and algD. This can be a novel strategy in the management of chronic wounds.

查看原文本刊更多论文

含有抗膜物质的聚-N-异丙基丙酰胺微凝胶对慢性伤口铜绿假单胞菌的影响

背景:生物膜的形成有助于铜绿假单胞菌(P. aeruginosa)在各种环境中生存。微凝胶可以有效地治疗细菌感染。本研究的主要目的是研究聚n -异丙基丙烯酰胺微凝胶(PNIPAM)对铜绿假单胞菌的作用。方法:从慢性伤口感染中分离到铜绿假单胞菌100株。进行了生物膜形成和抗生素敏感性的定量评估。进一步扩增algD、lasR和PA2714基因，研究基因频率和表达率。结果:edta处理后的样品中lasR表达明显降低。木糖醇处理显著降低了algD和lasR的表达。木糖醇处理后，PA2714的表达降低，但无统计学意义。结论:含木糖醇或EDTA的PNIPAM均能穿透铜绿假单胞菌的生物膜，显著降低lasR和algD的表达。这可能是一种治疗慢性伤口的新策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Avicenna journal of medical biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

2.90

自引率

0.00%

发文量