Exploring the role of fungal endophytes in the sudden death syndrome of the invasive shrub Chrysanthemoides monilifera subsp. rotundata in Australia

IF 3.3 3区 生物学 Q2 MICROBIOLOGY
L. Morin, Andrew Bissett, R. D. Klinken
{"title":"Exploring the role of fungal endophytes in the sudden death syndrome of the invasive shrub Chrysanthemoides monilifera subsp. rotundata in Australia","authors":"L. Morin, Andrew Bissett, R. D. Klinken","doi":"10.1094/pbiomes-04-21-0027-r","DOIUrl":null,"url":null,"abstract":"Pathogens that attack invasive plants can positively affect the integrity and functioning of ecosystems. Stem-tip dieback and extensive wilting followed by sudden death have been observed in Chrysanthemoides monilifera subsp. rotundata (bitou bush), one of Australia’s worst invasive shrubs. Metabarcoding and culturing methods were used to investigate if fungi are implicated in this syndrome. Metabarcoding results revealed significantly different endophytic fungal communities within healthy and diseased bitou bush, and co-located native plants. There was no difference in fungal communities between soil sampled in the root zone of healthy and diseased bitou bush at the same site. Two Diaporthe sp. operational taxonomic units (OTUs), dominant at sites with extensive wilting, explained 30% of the similarity between diseased bitou bush across all sites. Two other OTUs, Austropleospora osteospermi and Coprinellus sp., explained 20 and 40% of the similarity between diseased plants, respectively, and were only dominant at sites with dead or stunted, partially defoliated but not wilted bitou bush. A Penicillium sp. OTU explained 90% of the similarity between healthy bitou bush. Various Diaporthe spp. dominated isolations from diseased bitou bush. Manipulative experiments confirmed Diaporthe spp. pathogenicity on bitou bush excised and in-situ stems. In another experiment, Diaporthe masirevicii infected flowers and from there colonized stems endophytically, but wilting and sudden death of bitou bush did not occur within the experimental timeframe. Our study provides circumstantial evidence that bitou bush sudden death syndrome is the result of a shift in the composition of its endophytic fungal community, from mutualist to pathogenic species.","PeriodicalId":48504,"journal":{"name":"Phytobiomes Journal","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2021-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytobiomes Journal","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1094/pbiomes-04-21-0027-r","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Pathogens that attack invasive plants can positively affect the integrity and functioning of ecosystems. Stem-tip dieback and extensive wilting followed by sudden death have been observed in Chrysanthemoides monilifera subsp. rotundata (bitou bush), one of Australia’s worst invasive shrubs. Metabarcoding and culturing methods were used to investigate if fungi are implicated in this syndrome. Metabarcoding results revealed significantly different endophytic fungal communities within healthy and diseased bitou bush, and co-located native plants. There was no difference in fungal communities between soil sampled in the root zone of healthy and diseased bitou bush at the same site. Two Diaporthe sp. operational taxonomic units (OTUs), dominant at sites with extensive wilting, explained 30% of the similarity between diseased bitou bush across all sites. Two other OTUs, Austropleospora osteospermi and Coprinellus sp., explained 20 and 40% of the similarity between diseased plants, respectively, and were only dominant at sites with dead or stunted, partially defoliated but not wilted bitou bush. A Penicillium sp. OTU explained 90% of the similarity between healthy bitou bush. Various Diaporthe spp. dominated isolations from diseased bitou bush. Manipulative experiments confirmed Diaporthe spp. pathogenicity on bitou bush excised and in-situ stems. In another experiment, Diaporthe masirevicii infected flowers and from there colonized stems endophytically, but wilting and sudden death of bitou bush did not occur within the experimental timeframe. Our study provides circumstantial evidence that bitou bush sudden death syndrome is the result of a shift in the composition of its endophytic fungal community, from mutualist to pathogenic species.
探讨入侵灌木菊花(Chrysanthemoides monilifera subsp)猝死综合征中真菌内生菌的作用。澳大利亚的圆形数据
攻击入侵植物的病原体可以对生态系统的完整性和功能产生积极影响。菊花(Chrysanthemoides monilifera亚种)茎尖枯死和大面积萎蔫后突然死亡。圆刺灌木,澳大利亚最严重的入侵灌木之一。使用元条形码和培养方法来调查真菌是否与这种综合征有关。元条形码分析结果显示,健康和患病双头灌木以及同地原生植物的内生真菌群落存在显著差异。同一地点健康与患病双头灌木根区土壤间真菌群落差异无统计学意义。两个Diaporthe sp.的操作分类单位(OTUs)在广泛萎蔫的地点占主导地位,解释了所有地点中患病双头灌木之间30%的相似性。另外两个OTUs, Austropleospora osteospermi和Coprinellus sp,分别解释了患病植物之间20%和40%的相似性,并且仅在死亡或发育不良,部分落叶但未枯萎的灌木中占主导地位。一种青霉sp. OTU解释了健康双头灌木之间90%的相似性。从病灌木中分离出的分离物以各种散斑蝇为主。操作实验证实了该病菌对双灌木残茎和原位茎的致病性。在另一项实验中,大孢子虫内生感染花并从其定殖茎中侵染,但在实验时间范围内未发生双头灌木的萎蔫和猝死。我们的研究提供了间接证据,证明bitou bush猝死综合征是其内生真菌群落组成转变的结果,从共生物种到致病物种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
7.40
自引率
6.80%
发文量
42
审稿时长
4 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信