Potential bioremediation of heavy metal ions, polycyclic aromatic hydrocarbons and biofilms with South African hot spring bacteria

IF 1.9 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Bioremediation Journal Pub Date : 2022-03-06 DOI:10.1080/10889868.2021.1964429

J. Jardine

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引用次数: 4

Abstract

Abstract Hot spring bacteria, Bacillus and Bacillus-related spp. from South Africa were screened for biosorption and biosurfactant properties for bioremediation of toxic pollutants. Concentrations of heavy metal ions of copper, chromium, nickel and iron were determined spectrophotometrically. Biosorption resulted in reductions of chromium and copper with four isolates (7T, 9T, 30M, 83Li) but not for iron and nickel. The majority of 40 isolates were biosurfactant positive using paraffin oil. Twelve of which were further screened against petroleum and sunflower seed oil with only four showing positive in all assays tested (16S, 71T, 76S, 85Li). By LC-MS, two isolates (76S, 77S) were shown to produce biosurfactant, subtilisin. Nine biosurfactant-positive isolates were screened for their anti-biofilm properties. Brevibacillus sp. (16S) was able to disrupt a crystal violet stained biofilm of Bacillus subtilis (54T). Screening of bacterial isolates from hot springs reveals potential bacterial candidates with diverse biophysical properties that can be useful in bioremediation of hazardous polluted water.

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南非温泉细菌对重金属离子、多环芳烃和生物膜的潜在生物修复作用

摘要对来自南非的温泉细菌、芽孢杆菌和芽孢杆菌相关菌株进行了生物吸附和生物表面活性剂性能的筛选。用分光光度法测定了铜、铬、镍、铁等重金属离子的浓度。4个分离物(7T, 9T, 30M, 83Li)的生物吸附导致铬和铜的还原，但对铁和镍没有作用。40株分离菌中大部分石蜡油生物表面活性剂阳性。其中12种对石油和葵花籽油进行了进一步筛选，只有4种在所有测试中显示阳性(16S, 71T, 76S, 85Li)。液相色谱-质谱分析表明，76S、77S两株分离菌株可产生生物表面活性剂枯草杆菌素。筛选了9株表面活性剂阳性分离株的抗生物膜性能。短芽孢杆菌(16S)能够破坏枯草芽孢杆菌(54T)的结晶紫染色生物膜。从温泉中分离的细菌筛选揭示了具有不同生物物理性质的潜在候选细菌，可用于有害污染水的生物修复。

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来源期刊

Bioremediation Journal ENVIRONMENTAL SCIENCES-

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

9 months

期刊介绍： Bioremediation Journal is a peer-reviewed quarterly that publishes current, original laboratory and field research in bioremediation, the use of biological and supporting physical treatments to treat contaminated soil and groundwater. The journal rapidly disseminates new information on emerging and maturing bioremediation technologies and integrates scientific research and engineering practices. The authors, editors, and readers are scientists, field engineers, site remediation managers, and regulatory experts from the academic, industrial, and government sectors worldwide. High-quality, original articles make up the primary content. Other contributions are technical notes, short communications, and occasional invited review articles.