An investigation on cauda storage of sperm in hamster epididymis

Abstract

Immature testicular sperm of mammals acquire the potential to move in a forward direction during their journey through excurrent ducts, efferent ductules and the epididymis. The present study aimed to determine the sperm storage time using the hamster cauda epididymis. Ligation of the left epididymal tubule at the junction between the distal corpus and the proximal cauda was carried out to determine the storage time. The right epididymis was left unaltered as the control. On days 3, 12, 15, 24, 28, 32, and 40 after ligation of the cauda, experiments were carried out and repeated five times. Sperm total count and mortality were determined using the haemocytometer counting method and sperm viability was assessed with the live/dead viability kit. The morphology of cauda sperm was observed by preparing sperm smears from each animal. Data were analyzed using SPSS and all values were expressed as mean ± SEM. On day 40, the total number of cauda sperms was reduced remarkably. In the experimental groups and in the control, 3–6% of sperm motility was maintained until day 40. By day 3, the percentage of live sperm was 50% and by the 40th day, it was decreased up to 10%. In the control group, the live sperm percentage was 24% by the 40th day (). By day 32, 76% of the cauda spermatozoa appeared abnormal with head defects, mid piece and neck defects and multiple defects. Findings of the present study indicate that cauda sperm storage time is more than 40 days. Motility, viability and morphology of these spermatozoa were decreased remarkably during this storage time.