A simple and reliable assay to quantify host resistance and aggressiveness of the pathogen in the Fusarium head blight-barley pathosystem

N. Sakr, J. Al-Attar
{"title":"A simple and reliable assay to quantify host resistance and aggressiveness of the pathogen in the Fusarium head blight-barley pathosystem","authors":"N. Sakr, J. Al-Attar","doi":"10.1556/038.2022.00138","DOIUrl":null,"url":null,"abstract":"\n A simple and reliable method for quantifying Fusarium head blight (FHB), a widespread disease of barley, would enhance our capacity in identifying resistance sources and highly aggressive isolates. A detached head assay (DHA) was used to reliably assess: (i) resistance of two barley cultivars, Arabi Aswad (AS) and Arabi Abiad (AB) with different susceptibility to FHB and (ii) aggressiveness in a set of 16 fungal isolates of four Fusarium species. The two inoculated cultivars showed different responses in FHB incidence (DI) and severity (DS) using spray and point inoculation on detached barley heads, respectively. On AB, susceptible under several experimental conditions, inoculation with different Fusarium species resulted in significantly higher DI and DS, compared with AS, which showed Fusarium resistance. Furthermore, the values of DI and DS were significantly correlated with the previous findings generated under several experimental conditions. The use of this simple and reliable method in barley breeding programs can speed up the process of identification of sources of resistance to multiple FHB isolates. To our best knowledge, this is the first in-depth report investigating the usefulness of DHA for distinguishing susceptibility of barley plants and aggressiveness of diverse Fusarium species from a breeder's point of view.","PeriodicalId":7136,"journal":{"name":"Acta Phytopathologica Et Entomologica Hungarica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Phytopathologica Et Entomologica Hungarica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1556/038.2022.00138","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

A simple and reliable method for quantifying Fusarium head blight (FHB), a widespread disease of barley, would enhance our capacity in identifying resistance sources and highly aggressive isolates. A detached head assay (DHA) was used to reliably assess: (i) resistance of two barley cultivars, Arabi Aswad (AS) and Arabi Abiad (AB) with different susceptibility to FHB and (ii) aggressiveness in a set of 16 fungal isolates of four Fusarium species. The two inoculated cultivars showed different responses in FHB incidence (DI) and severity (DS) using spray and point inoculation on detached barley heads, respectively. On AB, susceptible under several experimental conditions, inoculation with different Fusarium species resulted in significantly higher DI and DS, compared with AS, which showed Fusarium resistance. Furthermore, the values of DI and DS were significantly correlated with the previous findings generated under several experimental conditions. The use of this simple and reliable method in barley breeding programs can speed up the process of identification of sources of resistance to multiple FHB isolates. To our best knowledge, this is the first in-depth report investigating the usefulness of DHA for distinguishing susceptibility of barley plants and aggressiveness of diverse Fusarium species from a breeder's point of view.
一种简单可靠的测定小麦枯萎病病原菌宿主抗性和侵袭性的方法
小麦赤霉病(Fusarium head blight, FHB)是一种广泛存在于大麦中的病害,建立一种简单可靠的定量方法将提高我们鉴定抗性来源和高侵袭性分离株的能力。采用分离头测定法(DHA)可靠地评估:(i)对赤霉病有不同敏感性的两个大麦品种Arabi Aswad (AS)和Arabi Abiad (AB)的抗性;(ii) 4种镰刀菌的16个真菌分离株的侵袭性。两个接种品种分别在离体大麦穗上采用喷雾接种和点接种,在FHB发病率(DI)和严重程度(DS)上表现出不同的反应。在几种试验条件下敏感的AB上,接种不同种类的镰刀菌,其DI和DS显著高于AS,表现出对镰刀菌的抗性。此外,在几种实验条件下,DI和DS的值与之前的研究结果显著相关。在大麦育种计划中使用这种简单可靠的方法可以加快对多种FHB分离株的抗性来源的鉴定过程。据我们所知,这是第一次从育种者的角度深入研究DHA对大麦植物的敏感性和不同镰刀菌物种的侵袭性的有用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Acta Phytopathologica Et Entomologica Hungarica
Acta Phytopathologica Et Entomologica Hungarica Agricultural and Biological Sciences-Plant Science
CiteScore
1.10
自引率
0.00%
发文量
7
期刊介绍: The journal publishes papers on the infectious diseases of plants, damages caused by insects and deals with the basic aspects of chemical and biological protection. Within its field of interest additional topics such as resistance against plant diseases, and physiological, biochemical and molecular questions of plant resistance and susceptibility are also discussed. Publishes book reviews and advertisements.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信