Effects of Grayanotoxin-III on different cell lines: in vitro ischemia model

Esin Akbay Çetin, Ç. Özenirler
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Abstract

Grayanotoxins (GTXs) are natural products and are mostly found in plants of the Ericaceae family, especially in the Rhododendron. With their ability to bind to voltage-gated sodium channels, they keep these channels constantly active and cause tissue damage. However, despite this feature, the use of Rhododendron leaves or its secondary products as an alternative product is especially common in Turkey. This study aims to evaluate the possible dose-related effects of GTX-III in ischemia-induced in vitro cell models. Within the scope of the study, an ischemia model was established in two different cell lines (H9c2 and Cos-7) and treated with various concentrations of GTX-III. In this context, cell viability, cytotoxicity, apoptosis and necrosis were examined. In the results of MTT, a significant decrease (+p < 0.05) in cell viability was observed in all GTX-III concentrations in H9c2 cells compared to the control, while a significant difference (+p < 0.05) was observed in Cos-7 cells, especially at the 24th hour. LDH cytotoxicity was increased in a dose-dependent manner in both cell models. It was concluded that GTX-III caused apoptosis, and reduced cell viability in ischemia models; however, promoted cell proliferation in healthy cells. Based on the literature review, this study is the first to document the cytotoxic properties and apoptotic potential of GTX-III in an in vitro cell culture ischemia model. Our findings support the usage of GTX-III, however it should be remembered that the dose needs to be verified before being used medically.
Grayanotoxin III对不同细胞系的影响:体外缺血模型
灰烷毒素(GTX)是天然产物,主要存在于Ericaceae科植物中,尤其是杜鹃花中。由于它们与电压门控钠通道结合的能力,它们使这些通道持续活跃并导致组织损伤。然而,尽管有这一特点,在土耳其,使用杜鹃花叶或其次级产品作为替代产品尤其常见。本研究旨在评估GTX-III在体外缺血诱导的细胞模型中可能的剂量相关作用。在研究范围内,在两种不同的细胞系(H9c2和Cos-7)中建立了缺血模型,并用不同浓度的GTX-III处理。在此背景下,检测了细胞活力、细胞毒性、细胞凋亡和坏死。在MTT的结果中,与对照相比,在H9c2细胞中的所有GTX-III浓度中观察到细胞活力的显著降低(+p<0.05),而在Cos-7细胞中观察到显著差异(+p<0.005),尤其是在第24小时。LDH细胞毒性在两种细胞模型中均呈剂量依赖性增加。结论是GTX-III在缺血模型中引起细胞凋亡,并降低细胞活力;然而促进了健康细胞中的细胞增殖。基于文献综述,本研究首次记录了GTX-III在体外细胞培养缺血模型中的细胞毒性和凋亡潜力。我们的研究结果支持GTX-III的使用,但应该记住的是,在进行医学使用之前,需要对剂量进行验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
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