Effect of Beauveria bassiana and Metarhizium anisopliae on different stages of Phlebotomus papatasi (Diptera: Psychodidae)

Maha Moustafa Ahmed, H. Mady, A. E. El Namaky
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引用次数: 0

Abstract

The sand fly, Phlebotomus papatasi (Scopoli, 1786) (Diptera: Psychodidae), is the main vector of Leishmania major Yakimoff and Schokhor, 1914, the causative agent of zoonotic cutaneous leishmaniasis North Africa, the Middle East, and North Sinai. The purpose of this study was to determine the effect of fungi on P. papatasi larvae, pupae, and adults using light microscopic analysis, scanning electron microscope (SEM), and histopathological studies. Specifically, larvae, pupae, and adult P. papatasi were infected with the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. Scanning electron microscope and histopathological methods were used to investigate the destructive impact of the fungi on the external and internal structures of P. papatasi. The results revealed propagation of the conidia on the cuticles of all P. papatasi life stages, including on the compound eyes, leg setae, thorax, wings, and abdomen of the adults. Histological sections of the control and treated larvae, pupae, and adults showed many alterations and malformations in the body and tissues of all life stages after 72 h. These results demonstrated that B. bassiana was more effective than M. anisopliae as a biological control of phlebotomine sand flies. Further studies to determine the best methods for delivery and application in the diverse ecological settings of the various leishmaniasis vectors are recommended.
球孢白僵菌和金龟子绿僵菌对不同阶段南瓜白蛉的防治作用
沙蝇,白蛉(Phlebotomus papatasi, Scopoli, 1786)(双翅目:白蛉科)是北非、中东和北西奈人畜共患皮肤利什曼病的病原体——主要利什曼原虫Yakimoff和Schokhor的主要媒介。本研究的目的是通过光镜分析、扫描电镜(SEM)和组织病理学研究来确定真菌对木瓜假蝇幼虫、蛹和成虫的影响。具体来说,木瓜假蝇的幼虫、蛹和成虫感染了球孢白僵菌和金龟子绿僵菌。采用扫描电镜和组织病理学方法研究了真菌对番木瓜外部和内部结构的破坏作用。结果表明,分生孢子在各生活期的表皮上均有繁殖,包括成虫复眼、腿毛、胸、翅和腹部。结果表明,球孢白僵菌对白蛉的生物防治效果优于金龟子白僵菌。短句来源建议进行进一步研究,以确定在各种利什曼病病媒的不同生态环境中提供和应用的最佳方法。
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来源期刊
Polish Journal of Entomology
Polish Journal of Entomology Agricultural and Biological Sciences-Insect Science
CiteScore
0.90
自引率
0.00%
发文量
11
期刊介绍: The Polish Journal of Entomology was founded in 1922 as a periodical of the Polish Entomological Society under the title Polskie Pismo Entomologiczne (subtitle Bulletin Entomologique de Pologne). The journal publishes original research papers and reviews from any entomological discipline. The Polish Journal of Entomology is sponsored by the Polish Entomological Society and the Ministry of Science and Higher Education. It deals with all aspects of entomology and has no geographical limits. Four issues of the journal are published annually. It is covered by the Zoological Record, Entomological Abstracts, Biological Abstracts. Each article has its own DOI
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