Insecticide Toxicity, Synergism and Resistance in Plutella xylostella (Lepidoptera: Plutellidae)

Acta Phytopathologica Et Entomologica Hungarica Pub Date : 2019-06-01 DOI:10.1556/038.54.2019.013

A. Soleymanzade, F. Khorrami, M. Forouzan

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引用次数: 3

Abstract

Plutella xylostella has become particularly notorious for its resistance to various insecticides. The toxicities of abamectin, hexaflumuron and indoxacarb to third instar larvae of the pest were assayed using the leaf-dipping method. The results showed that abamectin and indoxacarb with the lowest LC50 values exhibited stronger toxicity to larvae than hexaflumuron. To determine the synergism of PBO, DEM, DEF and TPP on the toxicity of tested insecticides and demonstrating possible biochemical mechanisms, an abamectin-, a hexaflu-muron- and an indoxacarb-resistant strain of P. xylostella were selected under laboratory conditions. After 10 generations of selection, the selected strains developed 14.21, 7.08, and 32.36-fold higher resistance to these insecticides, respectively. Abamectin resistance in abamectin-selected strain was suppressed with the synergists such as DEM and PBO, suggesting the involvement of monooxygeneses and glutathione S-transferase in the development of resistance in P. xylostella. Treatment with PBO and DEF significantly decreased the toxicity of hexaflumuron in the hexaflumuron-selected strain. Also, in indoxacarb-selected strain, the maximum synergism was occurred using PBO and DEF, followed by DEM and TPP. Hexaflumuron and indoxacarb synergism studies indicated in hexaflumuron resistance, monooxygenases and esterases, and in indoxacarb resistance, monooxygenases, esterases and glutathione S-transferae may be involved in the resistance mechanisms

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小菜蛾对杀虫剂的毒性、增效作用和抗性（鳞翅目：小菜蛾科）

小菜蛾因其对各种杀虫剂的抗性而臭名昭著。采用浸叶法测定了阿维菌素、六氟虫脒和吲唑威对三龄幼虫的毒性。结果表明，LC50值最低的阿维菌素和吲唑胺对幼虫的毒性比六氟虫隆强。为了确定PBO、DEM、DEF和TPP对测试杀虫剂毒性的协同作用，并证明可能的生化机制，在实验室条件下选择了一株阿维菌素、一株六流感muron和一株耐吲唑威的小菜蛾菌株。经过10代筛选，所选菌株对这些杀虫剂的抗性分别提高了14.21倍、7.08倍和32.36倍。DEM和PBO等增效剂抑制了阿维菌素筛选菌株对阿维菌素的抗性，表明单氧基因酶和谷胱甘肽S-转移酶参与了小菜蛾抗性的发展。PBO和DEF处理显著降低了六氟虫隆选定菌株中六氟虫琼的毒性。此外，在indoxacarb选择的菌株中，PBO和DEF的协同作用最大，其次是DEM和TPP。六氟脲和吲唑仑协同作用研究表明，在六氟脲抗性中，单加氧酶和酯酶，以及在吲唑唑抗性中，双加氧酶、酯酶和谷胱甘肽S-转移酶可能参与抗性机制

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来源期刊

Acta Phytopathologica Et Entomologica Hungarica Agricultural and Biological Sciences-Plant Science

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： The journal publishes papers on the infectious diseases of plants, damages caused by insects and deals with the basic aspects of chemical and biological protection. Within its field of interest additional topics such as resistance against plant diseases, and physiological, biochemical and molecular questions of plant resistance and susceptibility are also discussed. Publishes book reviews and advertisements.