Growth promotion potential of Bacillus spp. isolates on two tomato (Solanum lycopersicum L.) varieties in the West region of Cameroon

IF 1.8 Q2 AGRICULTURE, MULTIDISCIPLINARY

Open Agriculture Pub Date : 2023-01-01 DOI:10.1515/opag-2022-0154

Idriss Djoko Kouam, Joseline Mabah, Pierre Germain Ntsoli, Laverdure Tchamani, A. Yaouba, B. Katte, D. Bitom

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引用次数: 1

Abstract

Abstract Inoculation of plants with plant growth-promoting bacteria (PGPB) is an ecological approach to improve plants growth and yield. The objective of this study was to screen native PGPBs in tomato root rhizosphere and evaluate their performance in germination and growth of seedlings in the greenhouse in the western region of Cameroon. Isolates were selected based on their ability to solubilize phosphate, produce indole-3-acetic acid, and improve seed germination and seedling growth under laboratory conditions. Following this screening, eight bacteria were selected (S1, S4, S13, S14, S16, B9, B11, and B15) to assess their performance on seedling growth in greenhouse. Seeds of two tomato varieties (one improved, Raja, and the local one, Foolewouh) were used in this study. The experiment was performed in sterilized and non-sterilized soils. Phylogenetic analysis of the 16S rRNA sequence showed that these bacteria belong to the genus Bacillus, including B. thuringiensis, B. cereus, B. pacificus, and B. aerius. The bacterial performance depended on the variety and conditions of soil treatment. Isolates improved seed germination (up to 31.93%) and seeding strength (up to 78.59%) in vitro. In greenhouse, inoculation with PGPB significantly increased stem height, root length, and weight of fresh and dry stems and roots. Raja variety was more sensitive to bacteria and performed better than the Foolewouh variety. Bacterial isolates on sterilized soil allowed to better seedling growth compared to non-sterilized soil. The study showed that B. thuringiensis (strain B9), B. pacificus (strain B11), and Bacillus sp. (strain B15) can be used as inoculant formulations to improve seedling growth of tomato plant.

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芽孢杆菌分离株对喀麦隆西部两个番茄品种的促生长潜力

摘要植物生长促进菌(plant growth-promoting bacteria, PGPB)接种是一种促进植物生长和产量的生态途径。本研究的目的是筛选喀麦隆西部地区番茄根际原生PGPBs，并评价其对温室番茄幼苗萌发和生长的影响。在实验室条件下，根据其溶解磷酸盐、产生吲哚-3-乙酸和促进种子萌发和幼苗生长的能力来选择分离株。筛选8种细菌(S1、S4、S13、S14、S16、B9、B11和B15)，评价其对温室幼苗生长的影响。本研究使用了两种番茄品种的种子(一种是改良的Raja，另一种是当地的Foolewouh)。试验分别在灭菌和未灭菌的土壤中进行。16S rRNA序列的系统发育分析表明，这些细菌属于芽孢杆菌属，包括苏云金芽孢杆菌、蜡样芽孢杆菌、太平洋芽孢杆菌和aerius芽孢杆菌。细菌的表现取决于土壤处理的种类和条件。离体种子萌发率提高31.93%，种子萌发强度提高78.59%。在温室中，接种PGPB显著提高了鲜干茎的茎高、根长和根重。Raja品种对细菌更敏感，表现优于愚愚品种。与未灭菌土壤相比，灭菌土壤上的细菌分离株可以更好地促进幼苗生长。研究表明，苏云金芽孢杆菌(B9)、太平洋芽孢杆菌(B11)和芽孢杆菌(B15)可以作为接种剂，促进番茄幼苗生长。

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来源期刊

Open Agriculture AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

3.80

自引率

4.30%

发文量

审稿时长

9 weeks

期刊介绍： Open Agriculture is an open access journal that publishes original articles reflecting the latest achievements on agro-ecology, soil science, plant science, horticulture, forestry, wood technology, zootechnics and veterinary medicine, entomology, aquaculture, hydrology, food science, agricultural economics, agricultural engineering, climate-based agriculture, amelioration, social sciences in agriculuture, smart farming technologies, farm management.

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