Genetic analysis and identification of QTLs associated with yield-enhancing traits in Oryza sativa IR64 × Oryza glaberrima interspecific backcross populations

IF 1.8 4区农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY

Crop & Pasture Science Pub Date : 2023-01-06 DOI:10.1071/CP22105

Muralidhara Bharamappanavara, Manoj Appaiah Chikkahosahalli, B. Patil, Ajitha Vijjeswarapu, M. S. Anantha, Lokesha Ramappa, J. Diwan, J. Nidagundi, Umesh Rangappa Mathada, Suma Chandrasekhara Talagunda, Lokesh Yellaiah Guddalahalli, Rajanna Byanna, S. Rathod, R. Sundaram, L. V. Subbarao, Mahender Kumar Rapolu, C. Gireesh

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引用次数: 1

Abstract

ABSTRACT Context. Broadening the gene pool of staple food crops by incorporating desirable genes from wild/relative species is way to achieve higher genetic gains. The African rice species Oryza glaberrima Steud. is a source of genes for genetic improvement of Oryza sativa L. to various biotic and abiotic stresses. Aim. The study was undertaken to identify genomic regions associated with yield and yield-related traits in interspecific backcross mapping populations derived from variety IR64 (O. sativa indica) × O. glaberrima. Methods. The BC1F2 and BC1F2:3 populations were phenotyped for days to 50% flowering, plant height, number of tillers, number of productive tillers, panicle length, spikelet fertility and grain yield under irrigated conditions. The BC1F2 population was genotyped with 99 genome-wide polymorphic SSR markers. Quantitative trait loci (QTLs) associated with yield-enhancing traits were identified through single marker analysis. Key results. Significant genetic variability was observed with moderate to high heritability in mapping populations. Genotyping revealed the presence of segregation distortion and skewed genome composition. Single marker analysis identified 20 QTLs linked to the studied traits. A QTL linked to grain yield, qGY-4.1, derived from O. glaberrima was found in both populations with high phenotypic variance. The chromosome region around marker RM510 was identified as a QTL hotspot and putative candidate genes were identified. Conclusions. Our study demonstrates that O. glaberrima can serve as a valuable genetic resource for genetic improvement of yield-enhancing traits in O. sativa cultivars. Implications The identified QTLs regions can be employed in molecular breeding. The promising introgression lines identified can be used as pre-breeding lines in future breeding programs.

查看原文本刊更多论文

水稻IR64×水稻种间回交群体与增产性状相关QTL的遗传分析与鉴定

抽象的上下文。通过纳入野生/相对物种的优良基因来扩大主粮作物基因库是实现更高遗传收益的途径。非洲稻种glaberrima Steud。是水稻抗各种生物和非生物胁迫遗传改良的基因来源。的目标。本研究旨在鉴定品种IR64 (O. sativa indica) × O. glaberrima种间回交定位群体中与产量和产量相关性状相关的基因组区域。方法。在灌溉条件下，对BC1F2和BC1F2:3群体的开花天数~ 50%、株高、分蘖数、有效分蘖数、穗长、小穗育性和籽粒产量进行表型分析。用99个全基因组多态性SSR标记对BC1F2群体进行基因分型。通过单标记分析，鉴定出与增产性状相关的数量性状位点。关键的结果。在作图群体中观察到显著的遗传变异，遗传力中等至高。基因分型显示存在分离扭曲和基因组组成偏斜。单标记分析鉴定出与所研究性状相关的20个qtl。在两个表型变异较高的群体中均发现了一个与籽粒产量相关的QTL qGY-4.1。标记RM510周围的染色体区域被确定为QTL热点，并确定了可能的候选基因。结论。本研究结果表明，光斑草可作为一种宝贵的遗传资源，用于玉米品种增产性状的遗传改良。意义所鉴定的qtl区域可用于分子育种。鉴定出的有潜力的渐渗系可作为未来育种计划中的预育种系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop & Pasture Science AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

4.20

自引率

15.80%

发文量

111

审稿时长

3 months

期刊介绍： Crop and Pasture Science (formerly known as Australian Journal of Agricultural Research) is an international journal publishing outcomes of strategic research in crop and pasture sciences and the sustainability of farming systems. The primary focus is broad-scale cereals, grain legumes, oilseeds and pastures. Articles are encouraged that advance understanding in plant-based agricultural systems through the use of well-defined and original aims designed to test a hypothesis, innovative and rigorous experimental design, and strong interpretation. The journal embraces experimental approaches from molecular level to whole systems, and the research must present novel findings and progress the science of agriculture. Crop and Pasture Science is read by agricultural scientists and plant biologists, industry, administrators, policy-makers, and others with an interest in the challenges and opportunities facing world agricultural production. Crop and Pasture Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.