In vitro study on the effect of antibiotic combinations on Staphylococcus epidermidis biofilms

Q4 Medicine

Asian Journal of Ophthalmology Pub Date : 2020-04-30 DOI:10.35119/ASJOO.V17I2.533

Bhupinder Kaur, Shamim Ahmad, H. Ashraf, Giby Thachil

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Abstract

Purpose: Effect of combination of various antibiotics on Staphylococcus epidermidis biofilm. Study design: Experimental study. Methods: The biofilm-producing strains of S. epidermidis were isolated from 100 patients undergoing cataract surgery before instillation of any antibiotic. The strains were subjected to sensitivity test to various antibiotic combinations. The most effective agent was selected and its minimum inhibitory concentration was determined by broth dilution method. The statistics wereperformed using SPSS Version 23 (IBM Corp.) and Chi square test. Results: A total of 22 biofilm-positive samples were obtained. The combinations of vancomycin with ceftazidime (p < 0.05) followed by moxifloxacin with cefuroxime (p < 0.05) were found to be the most effective. Antibiofilm activity was also shown by other antibiotic combination. The minimum inhibitory concentration of vancomycin and ceftazidime in 11 samples was 2.5 and 2.8 mg/ml, while in the rest of the samples, it was 2.5 and 5.6 mg/ml, respectively. The minimum inhibitory concentration of moxifloxacin and cefuroxime was 0.125 and 2.81 mg/ml, respectively. Conclusion: In our study, we conclude that antibiotics are effective in eradicating biofilms.

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抗生素组合对表皮葡萄球菌生物膜影响的体外研究

目的:探讨多种抗生素联合应用对表皮葡萄球菌生物膜的影响。研究设计:实验研究。方法:从100例白内障手术患者中分离表皮葡萄球菌生膜菌，并对其进行抗生素治疗。对菌株进行了各种抗生素组合的敏感性试验。采用肉汤稀释法筛选出最有效的抑菌剂，并测定其最低抑菌浓度。统计使用SPSS Version 23 (IBM Corp.)和卡方检验。结果:共获得22份生物膜阳性样本。万古霉素与头孢他啶联用(p < 0.05)，莫西沙星与头孢呋辛联用(p < 0.05)效果最好。其他抗生素联合使用也显示出抗生物膜活性。11份样品中万古霉素和头孢他啶的最低抑菌浓度分别为2.5和2.8 mg/ml，其余样品中万古霉素和头孢他啶的最低抑菌浓度分别为2.5和5.6 mg/ml。莫西沙星和头孢呋辛的最低抑菌浓度分别为0.125和2.81 mg/ml。结论:抗生素对生物膜的清除是有效的。

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来源期刊

Asian Journal of Ophthalmology Medicine-Ophthalmology

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期刊介绍： Asian Journal of OPHTHALMOLOGY is the official peer-reviewed journal of the South East Asia Glaucoma Interest Group (SEAGIG) and is indexed in EMBASE/Excerpta Medica. Asian Journal of OPHTHALMOLOGY is published quarterly (four [4] issues per year) by Scientific Communications International Limited. The journal is published on-line only and is distributed free of cost via the SEAGIG website.