Synergistic antibacterial effects of Moroccan Artemisia herba alba, Lavandula angustifolia and Rosmarinus officinalis essential oils

Q2 Medicine

Synergy Pub Date : 2020-06-01 DOI:10.1016/j.synres.2019.100057

Imane Messaoudi Moussii , Kaotar Nayme , Mohammed Timinouni , Jamal Jamaleddine , Houda Filali , Farid Hakkou

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引用次数: 38

Abstract

Purpose

The antibacterial effects of three essential oils were tested against three bacterial strains (ATCC: Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) commonly known for their implication on wound healing infections.

Methods

The disc diffusion method and the determination of the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) were carried out to evaluate their antibacterial activity. The combined effect of essential oils against the tested bacteria was assessed by the checkerboard method and the Fractional Inhibitory Concentration Index (FICI) calculation.

Results

The results of the dissemination method demonstrated that the three EOs have antibacterial activity against the three tested bacteria at 20 μl.

Concerning the MIC, Lavender, Rosemary and Wormwood essential oils inhibited the bacterial growth at 1.33, 3.33 and 42.67 μl/ml; 1.33 1.67 and 42.67 μl/ml ; and 4.00, 6.67 and 42.67 μl/ml for S. aureus, E. coli and P. aeruginosa, respectively. The evaluation of the essential oils in combination revealed that the maximum of required concentration were 0.5 μl/ml for Artemisia herba alba and 1 μl/ml for Lavandula angustifolia and Rosmarinus officinalis.

Conclusions

These results indicate a synergism for the combination of lavender, wormwood and rosemary essential oils at very low concentrations compared to MICs.

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摩洛哥白蒿、薰衣草和迷迭香精油的协同抑菌作用

目的研究三种精油对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌三种常见的伤口愈合感染的抗菌作用。方法采用圆盘扩散法，测定其最低抑菌浓度(MIC)和最低杀菌浓度(MBC)，评价其抑菌活性。采用棋盘格法和分数抑菌浓度指数(FICI)计算法评价精油对被试细菌的综合抑菌效果。结果传菌实验结果表明，3种EOs对3种被试细菌均具有20 μl的抑菌活性。在MIC方面，薰衣草、迷迭香和艾草精油对细菌生长的抑制作用分别为1.33、3.33和42.67 μl/ml;1.33、1.67、42.67 μl/ml;金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌分别为4.00、6.67和42.67 μl/ml。复方精油评价结果表明，黄花蒿所需最大浓度为0.5 μl/ml，薰衣草和迷迭香所需最大浓度为1 μl/ml。结论与mic相比，薰衣草、艾草和迷迭香精油在极低浓度下联合使用具有协同作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Synergy Medicine-Medicine (miscellaneous)

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