Association between maternal dystocia and both the oxidant/antioxidant biomarkers and blood lactate in parturient Egyptian buffaloes (Bubalus bubalis)

Eslam Farag Fouad, Ahmed Saad Ahmed Hassaneen, Hassan Abdel-Sabour A. Hussein, A. M. H. Khalil, Nasra A. Yousef
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Abstract

Citation: Fouad et al., Association between maternal dystocia and both the oxidant/antioxidant biomarkers and blood lactate in parturient Egyptian buffaloes ( Bubalus bubalis ). 5(4): 1-14. Abstract Dystocia is a critical obstetrical problem in farm animals and is classified into maternal or fetal dystocia. The maternal causes of dystocia include different causes such as uterine torsion (UT), failure of cervical dilation (FCD), and uterine inertia (UI). Maternal dystocia induces massive oxidative stress due to increased production of free radicals with high levels of malondialdehyde (MDA). Little information is available on oxidants and antioxidant biomarkers and blood lactate (bLac) in parturient buffaloes with maternal dystocia. The current research aimed to clarify different causes of maternal dystocia in buffaloes and the association between the oxidative stress of parturient buffaloes and different types of maternal dystocia. Thirty-five parturient buffaloes were included in this study. Parturient buffaloes included normal parturition (NP; n=7), UT (n=23), FCD (n=4), and UI (n=1). Ten-ml blood samples were collected via jugular vein puncture into two tubes; plain vacutainer tubes for separation of serum to measure bLac and heparin coated vacutainer tube for separation of plasma for determination of MDA, glutathione peroxidase (GPx), and total antioxidant capacity (TAC). The results revealed that the major cause of maternal dystocia in the included buffaloes was UT. The UT was commonly post-cervical, clockwise, and > 180°. UT negatively affected both dam survival and calf viability. In addition, evaluation of oxidants and antioxidants biomarkers indicated that maternal dystocia causes more oxidative stress added to physiological stress of calving. This study found that, 4-days post-treatment period was not sufficient to resume antioxidant defense mechanism in UT buffaloes as no significant changes had been detected throughout the sampling period after treatment of UT. Moreover, evaluation of both GPx and bLac indicated their possible detection as indicators for prognosis of UT cases, where the concentration of GPx was significantly lower and the concentration of bLac was significantly higher in dead/culled buffaloes with UT.
埃及水牛(Bubalus bubalis)母体难产与氧化/抗氧化生物标志物和血乳酸的关系
引用:Fouad等人,产妇难产与分娩埃及水牛(Bubalus bubalis)的氧化剂/抗氧化剂生物标志物和血液乳酸之间的关系。5(4):1-14。摘要难产是农场动物的一个重要产科问题,可分为母体难产或胎儿难产。难产的母体原因包括不同的原因,如子宫扭转(UT)、宫颈扩张失败(FCD)和子宫惯性(UI)。母体难产由于自由基和高水平丙二醛(MDA)的产生增加而诱导大量氧化应激。关于母亲难产的分娩水牛的氧化剂、抗氧化生物标志物和血乳酸(bLac)的信息很少。目前的研究旨在阐明水牛产妇难产的不同原因,以及分娩水牛的氧化应激与不同类型产妇难产之间的关系。35头分娩水牛被纳入本研究。产程水牛包括正常分娩(NP;n=7)、UT(n=23)、FCD(n=4)和UI(n=1)。通过颈静脉穿刺将10ml血液样本收集到两个管中;用于分离血清以测定bLac的普通真空管和用于分离血浆以测定MDA、谷胱甘肽过氧化物酶(GPx)和总抗氧化能力(TAC)的肝素包被真空管。结果表明,超声检查是导致水牛难产的主要原因。超声检查常见于颈后、顺时针和>180°。UT对大坝存活率和小牛生存能力均产生负面影响。此外,对氧化剂和抗氧化剂生物标志物的评估表明,产妇难产导致更多的氧化应激,而不是产仔的生理应激。这项研究发现,治疗后4天不足以恢复UT水牛的抗氧化防御机制,因为在UT治疗后的整个采样期内没有检测到显著变化。此外,对GPx和bLac的评估表明,它们可能被检测为UT病例的预后指标,其中在具有UT的死/扑杀水牛中GPx的浓度显著较低,bLac的浓度显著较高。
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