Rosmarinus Improved Skin Flap Survival Through mTOR Dependent Pathway

Z. Javid, A. Norouzy, Zahra Bangaleh, Mahdi Shadnoush
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引用次数: 1

Abstract

Abstract Introduction: Skin flap application in the clinical practice is restricted due to the ischemic damage and flap necrosis. Rosmarinus oil has been shown to improve a skin flap survival. In the present work we studied the role of mammalian target of rapamycin (mTOR) signaling on rosmarinus-induced flap protection. Methods: A flap surgery was performed on Sprauge-Dawley rats (8 cm in by 3 cm). A week before and a week after the surgery the flaps were treated with topical rosmarinus oil (twice per day). Rapamycin (m-TOR inhibitor) was administered 30 minutes before the flap surgery in rosmarinus-treated or not treated groups. A week after the surgery the malondialdehyde (MDA) contents, myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, expression of Bax, Bcl-2, mTOR and p-mTOR were measured in the flap tissue. Results: Topical application of the rosmarinus increased the flap survival (p<0.05), anti-oxidative enzyme activity (SOD, p<0.05) and anti-apoptotic protein Bcl-2 expression. Rosmarinus treatment decreased the flap MDA content, MPO activity, and pro-apoptotic protein Bax expression (p<0.05). Rosmarinus topical application did not change mTOR expression and phosphorylation in the flap tissue. Expression of p-mTOR in rosmarinus treated group was suppressed by rapamycin pre-treatment, which also abolished rosmarinus effects on the flap survival (p<0.05). Conclusion: These data suggested p-mTOR dependent mechanism in rosmarinus-induced flap survival.
迷迭香通过mTOR依赖通路改善皮瓣存活
摘要简介:由于皮瓣缺血性损伤和坏死,皮瓣在临床上的应用受到限制。迷迭香油已被证明可以提高皮瓣的成活率。在本工作中,我们研究了哺乳动物雷帕霉素靶点(mTOR)信号在迷迭香诱导的皮瓣保护中的作用。方法:对Sprauge-Dawley大鼠(8cm×3cm)进行皮瓣手术。手术前一周和手术后一周,用迷迭香油局部治疗皮瓣(每天两次)。迷迭香治疗组和未治疗组在皮瓣手术前30分钟给予雷帕霉素(m-TOR抑制剂)。术后1周测定皮瓣组织中丙二醛(MDA)含量、髓过氧化物酶(MPO)和超氧化物歧化酶(SOD)活性、Bax、Bcl-2、mTOR和p-mTOR的表达。结果:局部应用迷迭香可提高皮瓣成活率(p<0.05)、抗氧化酶活性(SOD,p<0.05)和抗凋亡蛋白Bcl-2的表达。Rosmarinus治疗降低了皮瓣MDA含量、MPO活性和促凋亡蛋白Bax的表达(p<0.05)。Rosmarinu局部应用没有改变皮瓣组织中mTOR的表达和磷酸化。雷帕霉素预处理抑制了迷迭香治疗组p-mTOR的表达,也消除了迷迭香对皮瓣存活的影响(p<0.05)。结论:这些数据表明p-mTOR依赖于迷迭香诱导的皮瓣存活机制。
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期刊介绍: Serbian Journal of Dermatology and Venereology is a journal of the Serbian Association of Dermatologists and Venereologists. The journal is published in English, quarterly and intended to provide rapid publication of papers in the field of dermatology and venereology. Manuscripts are welcome from all countries in the following categories: editorials, original studies, review articles, professional articles, case reports, and history of medicine.
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