S. Srisuwatanasagul, Siriluck Jantautsa, S. Manee-in, Kongkiat Srisuwatanasagul, Rungrueang Yodsheewan, Amornthep Archawakulathep, P. Yamsakul, A. Roongsitthichai
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引用次数: 0
Abstract
The purpose of this study was to examine the expression of estrogen receptor alpha (ERα) in the testicular tissues of male fattening pigs injected with the first gonadotropin-releasing hormone (GnRH) vaccine (Improvac™, Zoetis, Thailand) for immunocastration 6 weeks earlier than the standard protocol. All pigs (n=24) were divided into three groups on the criterion of immunocastration protocol: A (n=8) was injected with GnRH vaccine at 15 and 19 weeks old, B (n=8) received GnRH vaccine at 9 and 19 weeks old, and C (n=8) remained untreated. Expression of ERα was investigated using an immunohistochemistry, appraised by an image analysis application (3DHISTECH, Budapest, Hungary), and reported as a H-score. The results revealed that testicular histoarchitecture of the immunocastrated pigs was less developed than that of the intact pigs. ERα was localized both in the seminiferous tubules and interstitial areas of all groups. ERα H-score of the C pigs was lowest (5.49±4.17) among groups and significantly lower than that of both A and B groups (P<0.05). However, the H-score of ERα between A and B groups was not different from each other (21.41±12.61 vs 23.91±11.47, P>0.05). In summary, the first injection of GnRH vaccine either at 9 or 15 weeks of age contributed to a similar result of testicular ERα expression.
本研究的目的是检测雄性育肥猪睾丸组织中雌激素受体α (ERα)的表达,这些育肥猪比标准方案提前6周注射了第一种促性腺激素释放激素(GnRH)疫苗(Improvac™,Zoetis,泰国)进行免疫去势。24头猪按免疫去势方案分为3组:A组(n=8)在15、19周龄注射GnRH疫苗,B组(n=8)在9、19周龄注射GnRH疫苗,C组(n=8)不注射。采用免疫组织化学方法检测ERα的表达,通过图像分析应用程序(3DHISTECH, Budapest, Hungary)进行评价,并以h评分报告。结果表明,免疫阉割猪的睾丸组织结构发育程度低于未阉割猪。ERα在各组精小管和间质区均有分布。C组ERα h评分最低(5.49±4.17),显著低于A组和B组(P0.05)。总之,在9周龄或15周龄时首次注射GnRH疫苗对睾丸ERα表达的影响相似。