Distribution and changes in the sbGnRH system in Rastrelliger brachysoma males during the breeding season

IF 0.8 4区生物学 Q4 MARINE & FRESHWATER BIOLOGY

Scientia Marina Pub Date : 2021-09-02 DOI:10.3989/scimar.05023.017

S. Senarat, J. Kettratad, W. Jiraungkoorskul, N. Kangwanrangsan, M. Amano, A. Shimizu, F. Plumley, Sasipong Tipdomrongpong

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引用次数: 0

Abstract

Rastrelliger brachysoma is a mariculture candidate species, but reproduction in captive fish has been problematic. This report examines the difference in the HPG axis, the neuroendocrine system and the development of reproductive tissues between captive vs. wild male R. brachysoma. The gonadosomatic index (GSI) of sexually mature male wild R. brachysoma was 1.12±0.34 and 1.94±0.26 during the non-breeding and breeding seasons, respectively. Captive R. brachysoma had a GSI of 1.88±0.17. All wild R. brachysoma were in the late spermatogenic stage irrespective of seasons. Immunostaining results showed that sbGnRH-immunoreactive neurons were distributed in three areas of the brain, namely the nucleus periventricularis, nucleus preopticus and nucleus lateralis tuberis. Follicle stimulating hormone and luteinizing hormone immunoreactivities were also observed in the pituitary gland. The levels of brain sbGnRH and GtH mRNA were not significantly different between the non-breeding and breeding seasons, but captive fish displayed (times or percent difference) lower mRNA levels than wild fish. These results suggest that these hormones control the testicular development in R. brachysoma and that the impaired reproduction in captivity may be due to their relative lower expression levels of follicle stimulating hormone and luteinizing hormone genes.

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繁殖季节短吻短吻龙雄蛛sbGnRH系统的分布和变化

短囊藻是海水养殖的候选物种，但在圈养鱼中的繁殖一直存在问题。本文研究了圈养雄性与野生雄性短鼻毛鼠在HPG轴、神经内分泌系统和生殖组织发育方面的差异。性成熟雄性野生短瘤田鼠在非繁殖期和繁殖期的性腺指数分别为1.12±0.34和1.94±0.26。圈养短鼻田鼠GSI为1.88±0.17。野生短裂田鼠在不同季节均处于生精后期。免疫染色结果显示，sbgnrh免疫反应神经元分布于脑室周围核、视前核和结节外侧核三个脑区。垂体中还观察到促卵泡激素和黄体生成素的免疫反应。非繁殖期和繁殖期鱼脑sbGnRH和GtH mRNA水平差异不显著，但圈养鱼的mRNA水平低于野生鱼(相差数倍或百分比)。这些结果表明，这些激素控制着短鼻毛囊鼠的睾丸发育，而圈养条件下繁殖受损可能是由于促卵泡激素和黄体生成素基因的表达水平相对较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Marina 生物-海洋与淡水生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Scientia Marina is the successor to Investigación Pesquera, a journal of marine sciences published since 1955 by the Institut de Ciències del Mar de Barcelona (CSIC). Scientia Marina is included in the Science Citation Index since 1998 and publishes original papers, reviews and comments concerning research in the following fields: Marine Biology and Ecology, Fisheries and Fisheries Ecology, Systematics, Faunistics and Marine Biogeography, Physical Oceanography, Chemical Oceanography, and Marine Geology. Emphasis is placed on articles of an interdisciplinary nature and of general interest.