Cell-Incorporated Bioactive Tissue Engineering Scaffolds made by Concurrent Cell Electrospinning and Emulsion Electrospinning

Pub Date : 2021-10-09 DOI:10.1142/s1793984421410051
Haoran Sun, Qilong Zhao, Liwu Zheng, W. Lu, Min Wang
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引用次数: 1

Abstract

Electrospun fibrous scaffolds attract great attention in tissue engineering owing to their high similarity in architecture to the extracellular matrix (ECM) that support cell attachment and growth in human bodies. Although they have shown superiority in promoting cell attachment and proliferation on their surfaces and hence, hold great promise for the regeneration of body tissues, the research still faces a great challenge of three-dimensional (3D) cell incorporation in electrospun scaffolds to form thick and cell-dense constructs because deep cell infiltration is hard to achieve in conventional electrospun scaffolds that normally have very small diameters of interconnected pores. Such hindrance has severely limited the clinical application of electrospun fibrous scaffolds to repair/regenerate various body tissues, particularly those with complex anatomies. To address this challenge, we have developed a concurrent cell electrospinning and emulsion electrospinning technique for fabricating bioactive bio-hybrid scaffolds with 3D and high-density cell incorporation. Through concurrent electrospinning, cell-encapsulated hydrogel fibers (“cell fibers”) and growth factor-containing ultrafine fibers are simultaneously deposited to form two-component scaffolds (i.e., scaffolds composed of two types of fibers) according to the design. With the breakup of cell fibers, live cells with well-preserved cell viability are released in situ inside the scaffolds, resulting in the creation of cell-incorporated bioactive scaffolds with ECM-mimicking fibrous architectures and 3D and high-density incorporation of cells. The growth and functions of incorporated cells in the scaffolds can be enhanced by the released growth factor from the emulsion electrospun fibrous component. The bioactive bio-hybrid scaffolds fabricated via concurrent electrospinning mimic the cell-matrix organization of body tissues and therefore have great potential for regenerating body tissues such as tendon and ligament.
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同时细胞静电纺丝和乳液静电纺丝制备细胞结合生物活性组织工程支架
电纺丝纤维支架由于其结构与支持人体细胞附着和生长的细胞外基质(ECM)高度相似,在组织工程中受到广泛关注。虽然它们在促进细胞在其表面的附着和增殖方面显示出优势,因此对身体组织的再生具有很大的希望,但由于传统的静电纺支架通常具有非常小直径的相互连接的孔,因此很难实现深层细胞浸润,因此研究仍然面临着三维(3D)细胞在静电纺支架中形成厚的和细胞密集的结构的巨大挑战。这种阻碍严重限制了电纺丝纤维支架在修复/再生各种人体组织,特别是复杂解剖结构组织中的临床应用。为了解决这一挑战,我们开发了一种同步细胞静电纺丝和乳液静电纺丝技术,用于制造具有3D和高密度细胞掺入的生物活性生物杂交支架。通过同步静电纺丝,将细胞包膜的水凝胶纤维(“细胞纤维”)和含有生长因子的超细纤维同时沉积,根据设计形成双组分支架(即由两种纤维组成的支架)。随着细胞纤维的断裂,保存完好的细胞活力的活细胞在支架内原位释放,从而产生细胞结合的生物活性支架,具有模拟ecm的纤维结构和三维高密度的细胞结合。乳状电纺丝纤维成分释放的生长因子可促进支架内细胞的生长和功能。通过同步静电纺丝制备的生物活性生物杂交支架模拟了人体组织的细胞-基质组织,因此在肌腱和韧带等身体组织再生方面具有很大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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