{"title":"Composition development and in vitro evaluation of O/W emulsions based on natural emulsifier Olivem 1000 as tea tree oil carriers","authors":"M. Wróblewska, K. Winnicka","doi":"10.32383/appdr/158784","DOIUrl":null,"url":null,"abstract":"The frequent occurrence of bacterial skin infections makes it necessary to search new solutions. Due to the limited effectiveness of traditional antibacterial agents, novel treatment alternatives are investigated. The use of plant origin compounds seems to be beneficial, because they are usually well tolerated and do not cause as many side effects as synthetic substances. Tea tree oil (TTO) is noted for well-established antimicrobial activity, so it can be used in the treatment of various skin diseases. It is effective against Gram-negative as well as Gram-positive bacteria. The aim of this work was to develop the composition and preparation technology of emulsion with TTO using natural emulsifier Olivem 1000 - combination of cetearyl olivate and sorbitan olivate. The designed formulations were evaluated by testing pH, stability and droplets size of the dispersed phase in the prepared emulsions. The type of emulsions was determined by selective phase staining method. In addition, rheological, mechanical, ex vivo bioadhesive properties of the prepared emulsions were studied and their antibacterial activity against selected strains was estimated. Obtained formulations were non-Newtonian systems, showing a shear-thinning behaviour and thixotropic properties, with proper textural features and beneficial bioadhesive properties. It was also demonstrated that formulations with lower viscosity showed higher antimicrobial efficacy against tested microorganisms and larger zones of inhibition were observed in case of Escherichia coli strains. The results of the study showed that the prepared emulsions appear to be a suitable form of topical administration of TTO.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta poloniae pharmaceutica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.32383/appdr/158784","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
The frequent occurrence of bacterial skin infections makes it necessary to search new solutions. Due to the limited effectiveness of traditional antibacterial agents, novel treatment alternatives are investigated. The use of plant origin compounds seems to be beneficial, because they are usually well tolerated and do not cause as many side effects as synthetic substances. Tea tree oil (TTO) is noted for well-established antimicrobial activity, so it can be used in the treatment of various skin diseases. It is effective against Gram-negative as well as Gram-positive bacteria. The aim of this work was to develop the composition and preparation technology of emulsion with TTO using natural emulsifier Olivem 1000 - combination of cetearyl olivate and sorbitan olivate. The designed formulations were evaluated by testing pH, stability and droplets size of the dispersed phase in the prepared emulsions. The type of emulsions was determined by selective phase staining method. In addition, rheological, mechanical, ex vivo bioadhesive properties of the prepared emulsions were studied and their antibacterial activity against selected strains was estimated. Obtained formulations were non-Newtonian systems, showing a shear-thinning behaviour and thixotropic properties, with proper textural features and beneficial bioadhesive properties. It was also demonstrated that formulations with lower viscosity showed higher antimicrobial efficacy against tested microorganisms and larger zones of inhibition were observed in case of Escherichia coli strains. The results of the study showed that the prepared emulsions appear to be a suitable form of topical administration of TTO.
期刊介绍:
The international journal of the Polish Pharmaceutical Society is published in 6 issues a year. The journal offers Open Access publication of original research papers, short communications and reviews written in English, in all areas of pharmaceutical sciences. The following areas of pharmaceutical sciences are covered: Analysis, Biopharmacy, Drug Biochemistry, Drug Synthesis, Natural Drugs, Pharmaceutical Technology, Pharmacology and General.
A bimonthly appearing in English since 1994, which continues “Acta Poloniae Pharmaceutica”, whose first issue appeared in December 1937. The war halted the activity of the journal’s creators. Issuance of “Acta Poloniae Pharmaceutica” was resumed in 1947. From 1947 the journal appeared irregularly, initially as a quarterly, then a bimonthly. In the years 1963 – 1973 alongside the Polish version appeared the English edition of the journal. Starting from 1974 only works in English are published in the journal. Since 1995 the journal has been appearing very regularly in two-month intervals (six books a year). The journal publishes original works from all fields of pharmacy, summaries of postdoctoral dissertations and laboratory notes.