Composition development and in vitro evaluation of O/W emulsions based on natural emulsifier Olivem 1000 as tea tree oil carriers

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY
M. Wróblewska, K. Winnicka
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引用次数: 0

Abstract

The frequent occurrence of bacterial skin infections makes it necessary to search new solutions. Due to the limited effectiveness of traditional antibacterial agents, novel treatment alternatives are investigated. The use of plant origin compounds seems to be beneficial, because they are usually well tolerated and do not cause as many side effects as synthetic substances. Tea tree oil (TTO) is noted for well-established antimicrobial activity, so it can be used in the treatment of various skin diseases. It is effective against Gram-negative as well as Gram-positive bacteria. The aim of this work was to develop the composition and preparation technology of emulsion with TTO using natural emulsifier Olivem 1000 - combination of cetearyl olivate and sorbitan olivate. The designed formulations were evaluated by testing pH, stability and droplets size of the dispersed phase in the prepared emulsions. The type of emulsions was determined by selective phase staining method. In addition, rheological, mechanical, ex vivo bioadhesive properties of the prepared emulsions were studied and their antibacterial activity against selected strains was estimated. Obtained formulations were non-Newtonian systems, showing a shear-thinning behaviour and thixotropic properties, with proper textural features and beneficial bioadhesive properties. It was also demonstrated that formulations with lower viscosity showed higher antimicrobial efficacy against tested microorganisms and larger zones of inhibition were observed in case of Escherichia coli strains. The results of the study showed that the prepared emulsions appear to be a suitable form of topical administration of TTO.
以天然乳化剂Olivem 1000为茶树油载体的油水乳状液的组成及体外评价
细菌性皮肤感染的频繁发生使得有必要寻找新的解决方案。由于传统抗菌药物的效果有限,新的治疗方案正在研究中。使用植物来源的化合物似乎是有益的,因为它们通常耐受性良好,不会像合成物质那样产生许多副作用。茶树油(TTO)以其良好的抗菌活性而闻名,因此它可用于治疗各种皮肤病。它对革兰氏阴性菌和革兰氏阳性菌都有效。研究了以天然乳化剂Olivem - 1000为原料,以棕榈醇酯和山梨醇酯为复配物,制备含TTO乳液的配方和工艺。通过测试所制乳剂的pH值、分散相的稳定性和液滴大小来评价所设计的配方。采用选择相染色法测定乳剂类型。此外,还研究了制备的乳剂的流变学、力学和离体生物粘附性能,并对所选菌株进行了抑菌活性评价。获得的配方是非牛顿体系,具有剪切变薄行为和触变性能,具有适当的纹理特征和有益的生物粘附性能。实验还表明,黏度越低的配方对微生物的抑菌效果越好,对大肠杆菌的抑菌效果也越明显。研究结果表明,所制备的乳剂似乎是局部给药的一种合适的形式。
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来源期刊
CiteScore
0.80
自引率
0.00%
发文量
74
审稿时长
6-12 weeks
期刊介绍: The international journal of the Polish Pharmaceutical Society is published in 6 issues a year. The journal offers Open Access publication of original research papers, short communications and reviews written in English, in all areas of pharmaceutical sciences. The following areas of pharmaceutical sciences are covered: Analysis, Biopharmacy, Drug Biochemistry, Drug Synthesis, Natural Drugs, Pharmaceutical Technology, Pharmacology and General. A bimonthly appearing in English since 1994, which continues “Acta Poloniae Pharmaceutica”, whose first issue appeared in December 1937. The war halted the activity of the journal’s creators. Issuance of “Acta Poloniae Pharmaceutica” was resumed in 1947. From 1947 the journal appeared irregularly, initially as a quarterly, then a bimonthly. In the years 1963 – 1973 alongside the Polish version appeared the English edition of the journal. Starting from 1974 only works in English are published in the journal. Since 1995 the journal has been appearing very regularly in two-month intervals (six books a year). The journal publishes original works from all fields of pharmacy, summaries of postdoctoral dissertations and laboratory notes.
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