Diversidad de haplotipos del complejo principal de histocompatibilidad en equinos de la raza Árabe de la República Argentina

Sebastian Sadaba, C. C. Botto, María Eugenia Zappa, M. Carino, E. E. V. Castagnasso, P. P. García, S. Díaz
{"title":"Diversidad de haplotipos del complejo principal de histocompatibilidad en equinos de la raza Árabe de la República Argentina","authors":"Sebastian Sadaba, C. C. Botto, María Eugenia Zappa, M. Carino, E. E. V. Castagnasso, P. P. García, S. Díaz","doi":"10.24215/15142590E003","DOIUrl":null,"url":null,"abstract":"Genetic diversity of the major histocompatibility complex (MHC) is essential for the antigen recognition and presentation during the immune response. Indirect MHC typing by microsatellites (STR) provides data on genetic diversity and population structure, and provides knowledge of selective and evolutionary processes. In order to characterize a sample of Arab horses, we identified MHC haplotypes based on three STRs (UM011, DRB2-STR2 and COR112) covering a region of ~1Mpb. Genomic DNA was extracted from blood and hair of 30 horses from five farms of the province of Buenos Aires. STRs were amplified with fluorescent primers and typed in an automated sequencer. Population and diversity parameters were estimated. Haplotype detection was performed by segregation analysis and with the PHASE program for reconstructing haplotypes. Of the 24 haplotypes identified, 12 were verified by segregation in the Stud Book pedigree records. Our results showed linkage with known equine leukocyte antigen (ELA) class II alleles. The number of haplotypes identified allowed to expand the estimates of diversity in the equine MHC. This methodology is a useful tool and an alternative approach to type MHC in horse lineages and immune-related population studies.","PeriodicalId":7773,"journal":{"name":"Analecta Veterinaria","volume":"37 1","pages":"003-003"},"PeriodicalIF":0.0000,"publicationDate":"2017-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analecta Veterinaria","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24215/15142590E003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Genetic diversity of the major histocompatibility complex (MHC) is essential for the antigen recognition and presentation during the immune response. Indirect MHC typing by microsatellites (STR) provides data on genetic diversity and population structure, and provides knowledge of selective and evolutionary processes. In order to characterize a sample of Arab horses, we identified MHC haplotypes based on three STRs (UM011, DRB2-STR2 and COR112) covering a region of ~1Mpb. Genomic DNA was extracted from blood and hair of 30 horses from five farms of the province of Buenos Aires. STRs were amplified with fluorescent primers and typed in an automated sequencer. Population and diversity parameters were estimated. Haplotype detection was performed by segregation analysis and with the PHASE program for reconstructing haplotypes. Of the 24 haplotypes identified, 12 were verified by segregation in the Stud Book pedigree records. Our results showed linkage with known equine leukocyte antigen (ELA) class II alleles. The number of haplotypes identified allowed to expand the estimates of diversity in the equine MHC. This methodology is a useful tool and an alternative approach to type MHC in horse lineages and immune-related population studies.
在免疫应答过程中,主要组织相容性复合体(MHC)的遗传多样性对抗原识别和递呈至关重要。微卫星间接MHC分型提供了遗传多样性和群体结构的数据,并提供了选择和进化过程的知识。为了表征阿拉伯马样本,我们基于三个STRs (UM011, DRB2-STR2和COR112)确定了MHC单倍型,覆盖了约1Mpb的区域。研究人员从布宜诺斯艾利斯省5个农场的30匹马的血液和毛发中提取了基因组DNA。用荧光引物扩增STRs,并在自动测序仪上进行分型。估计了种群和多样性参数。单倍型检测采用分离分析和PHASE程序进行单倍型重建。在鉴定的24个单倍型中,有12个在种马书系谱记录中得到了分离验证。我们的结果显示与已知的马白细胞抗原(ELA) II类等位基因连锁。确定的单倍型数量允许扩大对马MHC多样性的估计。这种方法是一种有用的工具,也是马谱系和免疫相关人群研究中MHC型的替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
0.30
自引率
0.00%
发文量
4
审稿时长
24 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信