Genetic diversity and population structure of Irvingia species using DArTseq generated markers

IF 1.1 Q4 ENVIRONMENTAL STUDIES

Forests, Trees and Livelihoods Pub Date : 2022-12-22 DOI:10.1080/14728028.2022.2160380

Sylvia Jepkemboi, Patroba Ojola

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引用次数: 0

Abstract

ABSTRACT Irvingia gabonensis and Irvingia wombolu trees indigenous to West and Central Africa are economically important owing to their valuable kernels. Massive fruit harvesting and reduction of forests land area has threatened their genetic diversity thus warranting conservation. This study reports the genetic diversity and population structure based on dominant markers of Irvingia accessions from Nigeria, Cameroon and Gabon held at World Agroforestry field genebanks. A total of 60 samples and 6532 SilicoDArT markers revealed high genetic differentiation among populations (phiPT = 0.301, P = .001). The overall genetic diversity revealed by Nei’s gene diversity (He = 0.117) and percentage of polymorphic loci (39.60%) was low. I.gabonensis provenances from Cameroon and Nigeria were more diverse than Gabonese populations. Pairwise phiPT values calculated from AMOVA variances delineated I. wombolu and I. gabonensis. Population structure and cluster analysis based on Bayesian algorithm, DAPC and UPGMA methods placed Cameroon at the center of distribution of I. gabonensis accessions from Nigeria and Gabon. Gabonese accessions tend to cluster independent of Nigerian accessions while I. wombolu formed a distinct cluster from I. gabonensis. SilicoDArT markers were found suitable for population structure and differentiation analysis of Irvingia accessions and can guide future recollections to enrich genebank diversity and materials for domestication events.

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应用DArSeq标记研究Irvingia种的遗传多样性和种群结构

原产于西非和中非的加蓬欧文树(Irvingia gabonensis)和温博鲁欧文树(Irvingia wombolu)因其珍贵的籽粒而具有重要的经济价值。大量的水果采收和森林土地面积的减少已经威胁到它们的遗传多样性，因此需要保护。本研究报告了世界农林业大田基因库中来自尼日利亚、喀麦隆和加蓬的Irvingia种质资源的遗传多样性和群体结构。60个样本和6532个标记显示群体间存在较高的遗传分化(phiPT = 0.301, P = .001)。Nei’s基因多样性(He = 0.117)和多态性位点百分比(39.60%)显示的总体遗传多样性较低。来自喀麦隆和尼日利亚的加蓬瓢虫种源比加蓬瓢虫种群更多样化。根据AMOVA方差计算出的两两phiPT值描绘了温博鲁和加蓬沙鼠。基于贝叶斯算法、DAPC和UPGMA的种群结构和聚类分析表明，喀麦隆是尼日利亚和加蓬加蓬蠓种群分布的中心。加蓬的物种倾向于独立于尼日利亚的物种而I. wombolu形成了一个与I. gabonensis不同的集群。发现silodart标记适用于Irvingia品种的群体结构和分化分析，可以指导未来的回忆，以丰富基因库的多样性和驯化事件的材料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Forests, Trees and Livelihoods Agricultural and Biological Sciences-Forestry

CiteScore

2.20

自引率

0.00%

发文量

期刊介绍： Forests, Trees and Livelihoods originated in 1979 under the name of the International Tree Crops Journal and adopted its new name in 2001 in order to reflect its emphasis on the diversity of tree based systems within the field of rural development. It is a peer-reviewed international journal publishing comments, reviews, case studies, research methodologies and research findings and articles on policies in this general field in order to promote discussion, debate and the exchange of information and views in the main subject areas of.