In vitro regeneration of Haloxylon ammodendron

Abstract

Haloxylon ammodendron (C.A.Mey) Bunge is one of the important species of arid desert vegetation in China, and it is also an ideal material for studying plant stress resistance, which plays an extremely important role in protecting desert ecosystems and preventing land desertification. However, there are relatively few studies on the regeneration of the fusiform, and the genetic transformation system has not been successfully reported, which restricts the in-depth study of the molecular mechanism of shuttle propagation and stress resistance. In the present study, the seeds, hypocotyls, cotyledons, cotyledon nodes, terminal buds and fixed buds were used as explants, and a set of tissue culture and plant regeneration system was established by inducing adventitious buds, adventitious bud rooting and transplanting. The results showed that amongst different H. ammodendron explants, i.e., seeds, hypocotyls, cotyledons and cotyledon nodes, the last induced budding effect was better. The optimal medium for inducing clandine buds by cotyledon segment differentiation is 0.5 mg·L-1 NAA+0.5 mg·L-1 6-BA induced budding rate was high, reaching 61.90%, rooting medium was 1/2 MS+1 mg·L-1 NAA +1 mg·L-1 IBA + 1 mg·L-1 IAA with a rooting rate of 50%. The results of this study will provide a theoretical basis for the genetic transformation of H. ammodendron.