Isolation and evaluation of biological properties and differentiation potential of human umbilical cord-derived mesenchymal stem cells in covid-19 positive patients

Laplage em Revista Pub Date : 2021-10-14 DOI:10.24115/s2446-6220202173d1745p.542-551

S. Babaei, M. Mirzaei, Narges Maleki, Z. Alizadeh, Fahimeh Yasari, M. Novin

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Abstract

The present study investigated the biological properties of umbilical cord-derived mesenchymal stem cells and their ability to differentiate in patients who have had positive COVID-19 PCR test result during their pregnancies. In this experimental laboratory study, the whole placenta specimens were obtained from 30 positive COVID-19 women undergoing cesarean section, with age 20 to 40 years, and kept under standardized conditions. The mesenchymal cells were isolated by enzymatic method and their morphological characteristics were examined by microscopy and absorption spectroscopy and their biological properties, in particular expression of CD markers, were determined by flow cytometry. Finally, mesenchymal stem cells were cultured in specific media in order to differentiate into osteocyte and adipocyte. Data were analyzed using descriptive statistics. Morphological and physical examinations by microscope and absorption spectroscopy as well as presenting of CD44, CD73, CD90, and CD105 markers and lacking CD34 and CD45 markers demonstrated the mesenchymal entity of stem cells. Mesenchymal stem cells successfully differentiated into osteocyte and adipocyte.

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新冠肺炎阳性患者脐血间充质干细胞生物学特性及分化潜能的分离评价

本研究调查了妊娠期间新冠肺炎PCR检测结果呈阳性的患者的脐带源性间充质干细胞的生物学特性及其分化能力。在这项实验性实验室研究中，整个胎盘标本取自30名新冠肺炎阳性剖宫产妇女，年龄在20至40岁之间，并在标准化条件下保存。用酶法分离间充质细胞，用显微镜和吸收光谱法检测其形态特征，用流式细胞术测定其生物学特性，特别是CD标记物的表达。最后，将间充质干细胞在特定培养基中培养，以分化为骨细胞和脂肪细胞。使用描述性统计对数据进行分析。通过显微镜和吸收光谱进行的形态学和物理检查，以及CD44、CD73、CD90和CD105标记物的存在和缺乏CD34和CD45标记物，证明了干细胞的间充质实体。间充质干细胞成功分化为骨细胞和脂肪细胞。

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Laplage em Revista EDUCATION & EDUCATIONAL RESEARCH-

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8 weeks