Prevalence, Molecular Identification, Antimicrobial Resistance, and Disinfectant Susceptibility of Listeria innocua Isolated from Ready-to-Eat Foods Sold in Johannesburg, South Africa

Q3 Agricultural and Biological Sciences

Journal of Food Quality and Hazards Control Pub Date : 2021-09-10 DOI:10.18502/jfqhc.8.3.7200

H. Makumbe, F. Tabit, B. Dlamini

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引用次数: 2

Abstract

Background: Food contamination with Listeria spp. can occur at all stages of the food chain. The aim of this research was to investigate the prevalence, molecular identification, antimicrobial resistance, and disinfectant susceptibility of Listeria innocua isolated from Ready-To-Eat (RTE) foods sold in Johannesburg, South Africa. Methods: Eighty RTE foods were collected from Johannesburg, South Africa. The 16S rRNA region of L. innocua isolates was amplified, sequenced, and identified using Basic Alignment Search Tool (BLAST). The antimicrobial resistance and disinfectant susceptibility (against four commercial disinfectants) of the isolates were evaluated using disk diffusion and microdilution assays. Data were statistically analyzed using SPSS v. 23.0. Results: Listeria strains revealed a high 16S rRNA gene sequence analogy to L. innocua of between 98-99%. The overall prevalence of L. innocua was 21.3% (17 out of 80) in the RTE food samples. Most isolates were susceptible to the studied commercial disinfectants. All the L. innocua isolates from food sources were found to be resistant to ampicillin and cephalothin, while 83 and 74% of isolates were resistant to colistin sulphate and sulphatriad. Conclusion: Prevalence of L. innocua was considerable in the RTE food samples sold in Johannesburg, South Africa. The L. innocua isolates showed high antibiotic resistance against ampicillin, cephalothin, colistin sulphate, and sulphatriad.

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南非约翰内斯堡出售的即食食品中分离的无菌性李斯特菌的流行、分子鉴定、耐药性和消毒剂敏感性

背景:食品污染李斯特菌可发生在食物链的所有阶段。本研究的目的是调查从南非约翰内斯堡销售的即食食品(RTE)中分离的无性李斯特菌的流行程度、分子鉴定、抗微生物药物耐药性和消毒剂敏感性。方法:从南非约翰内斯堡收集80种RTE食品。利用BLAST扩增、测序和鉴定了innocul菌株的16S rRNA区域。采用纸片扩散法和微量稀释法对4种市售消毒剂的耐药性和敏感性进行评价。数据采用SPSS v. 23.0进行统计学分析。结果:李斯特菌的16S rRNA基因序列与L. innocua相似度在98 ~ 99%之间。在RTE食品样品中，innocua菌的总体流行率为21.3%(80份样品中有17份)。大多数分离株对所研究的市售消毒剂敏感。从食物来源分离出的无性乳杆菌对氨苄西林和头孢菌素均有耐药性，而对硫酸粘菌素和硫酸三酸酯分别有83%和74%的菌株耐药。结论:南非约翰内斯堡销售的RTE食品样品中无尾乳杆菌的流行率较高。该菌株对氨苄西林、头孢菌素、硫酸粘菌素和磺胺吡啶具有较高的耐药性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Food Quality and Hazards Control Agricultural and Biological Sciences-Food Science

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of Food Quality and Hazards Control (J. Food Qual. Hazards Control) is an international peer-reviewed quarterly journal that aims at publishing of high quality articles involved in food quality, food hygiene, food safety, and food control which scientists from all over the world may submit their manuscript. This academic journal aims to improve international exchange of new findings and recent developments in all aspects of agricultural and biological sciences. This free of charge journal is published in both online and print forms and welcomes the manuscripts that fulfill the general criteria of novelty and scientific importance. Among the most significant objectives of Journal of Food Quality and Hazards Control are to ensure that the articles reflect a wide range of topics regarding journal scopes; to do a fair, scientific, fast, as well as high quality peer-review process; to provide a wide and diverse geographical coverage of articles around the world; and to publish the articles having a trustable resource of scientific information for the audiences. The types of acceptable submissions include original article, review article, short communication, letter to the editor, case report, editorial, as well as book review. Journal of Food Quality and Hazards Control is an official journal of Research Center for Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.