Efficacy of disinfectants on microbial contaminated toothbrushes

Scientific Dental Journal Pub Date : 2019-09-01 DOI:10.4103/SDJ.SDJ_23_19

Angelica Tiara, A. Widyarman, C. Rovani

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引用次数: 2

Abstract

Background: The storage condition of a toothbrush can influence the growth of bacteria. Indonesia as a developing country has a low awareness of the importance of toothbrush hygiene. Thus, an efficient and economic toothbrush storage solution is needed. Objective: The aim of this study was to evaluate the effect of soaking toothbrushes in mouthwash solution on the total number of Streptococcus mutans and Fusobacterium on the toothbrush. Methods: Eighteen toothbrushes were provided to healthy individuals aged 19–23-years. For 1 month, each toothbrush was soaked in disinfectant solution (25 mL) for 20 min after brushing, with Group 1 using mouthwash and Group 2 using sterile tap water. The real-time polymerase chain reaction (PCR) method was used to evaluate the number of microorganisms on each toothbrush. The total number of DNA targets was identified using real-time PCR followed by SYBR Green reagents and 16S rRNA-gene specific primers for S. mutans and Fusobacterium. A paired sample t-test was used for statistical analysis, and the level of significance was set at P < 0.05. Results: There were significant differences in the total bacterial numbers of S. mutans and Fusobacterium on toothbrushes after soaking with mouthwash solution. The average scores (Log10-CFU/mL ± standard deviation) of the total bacteria (2.66 ± 0.39), S. mutans (1.21 ± 0.18), and Fusobacterium (10.35 ± 6.02) on toothbrushes in Group 1 were significantly decreased compared to the average scores of the total bacterial load (5.19 ± 0.41), S. mutans (2.71 ± 1.59), and Fusobacterium (18.96 ± 4.26) in Group 2. Statistical evaluation brought statistically significant difference of total bacteria numbers and S. mutans between Group 1 and Group 2 (P < 0.05). Conclusion: Soaking toothbrushes in mouthrinse solution reduce the total number of bacterial load, S. mutans, and Fusobacterium. Further studies are needed to explore the effects of mouthwash solution against other oral pathogens.

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消毒剂对受微生物污染牙刷的疗效

背景：牙刷的储存条件会影响细菌的生长。印度尼西亚作为一个发展中国家，对牙刷卫生的重要性认识不高。因此，需要一种高效且经济的牙刷储存解决方案。目的：评价漱口液浸泡牙刷对牙刷上变形链球菌和梭杆菌总数的影响。方法：向19-23岁的健康人提供18支牙刷。在1个月内，每把牙刷在刷牙后浸泡在消毒溶液（25mL）中20分钟，第1组使用漱口水，第2组使用无菌自来水。使用实时聚合酶链式反应（PCR）方法来评估每把牙刷上的微生物数量。使用实时PCR、SYBR Green试剂和变异链球菌和梭杆菌的16S rRNA基因特异性引物鉴定DNA靶标的总数。采用配对样本t检验进行统计分析，显著性水平设定为P<0.05。结果：漱口液浸泡牙刷后，牙刷上的变形链球菌和梭杆菌总数存在显著差异。第1组牙刷上总细菌（2.66±0.39）、变异链球菌（1.21±0.18）和梭杆菌（10.35±6.02）的平均得分（Log10CFU/mL±标准差）与第2组总细菌载量（5.19±0.41）、变异链霉菌（2.71±1.59）和梭菌（18.96±4.26）的平均分数相比显著降低。统计评价显示，第1组和第2组的细菌总数和变异链球菌数量差异有统计学意义（P<0.05）。结论：牙刷浸泡在漱口液中可减少细菌载量、变异链球菌和梭杆菌的总数。还需要进一步的研究来探索漱口水溶液对其他口腔病原体的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientific Dental Journal

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0.00%

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审稿时长

7 weeks