Anatomopathological, ultrastructural, immunohistochemical and molecular characterization of infectious laryngotracheitis outbreaks in poultry farms in Egypt (2018–2020)

Q4 Veterinary
M. El-Saied, M. El-Mahdy, Ezz El-Din Sakr, M. Bastami, M. Shaalan
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引用次数: 2

Abstract

Infectious laryngotracheitis (ILT) is a severe respiratory disease, which causes high morbidity and mortality in affected birds. In our study, ILT were reported in 42 farms from nine governates over two years (2018–2020) that showed clinical signs of ILT including dyspnea, blood expectoration of, excessive lacrimation, rattling, conjunctivitis. The disease affected different chicken breeds and age groups despite vaccination with licensed and commonly used vaccines. Samples of larynx, trachea, lungs and air sacs were examined and collected for histopathological, ultrastructural, immunohistochemical examination and molecular detection. Gross examination of laryngeal and tracheal lumen revealed different types of exudate varied from catarrhal to fibrinonecotric, also pneumonia and airsacculitis were detected. Histopathological examination showed different alternation in larynx, trachea, lung and air sac as characteristic syncytial cells containing intranuclear inclusion body hanged in fibrinoheterphilic exudate that precent in laryngeal, tracheal, bronchial and parabronchial lumen and air sacs. Tracheal lesion scoring system was used to categorize the severity of lesion in different governates. Tracheal lesion score showed that 6.02%, 26.5%, 43.3% of the birds exhibited mild, moderate, and severe changes, respectively, while 24.18% of the birds exhibited very severe changes. Furthermore, severe cases were related to the Qalyubia , Fayoum then Sharkia Governorate. Moreover, immunohistochemistry was used to detect viral particles in syncytial cells, inflammatory cells beside epithelium of trachea and lung. Transmission electron microscopy enabled the detection of virus particles and demonstrated that heterophils could be infected. PCR targeting a region in the thymidine kinase gene and glycoprotein gJ gene confirmed the presence of infectious laryngotracheitis ILT virus-specific DNA. In conclusion, anatomopathological, immunohistochemical, molecular and ultrastructural findings showed increased of ILTV severity in Egypt. Larynx, trachea, lungs and air sac should be collected and examined that aid in diagnosis. Importance of good biosecurity level to be considered.
2018-2020年埃及家禽场传染性喉气管炎暴发的解剖病理、超微结构、免疫组织化学和分子特征
传染性喉气管炎(ILT)是一种严重的呼吸道疾病,在患病鸟类中引起高发病率和高死亡率。在我们的研究中,在两年(2018-2020)的时间里,来自9个省的42个农场报告了ILT,这些农场表现出ILT的临床症状,包括呼吸困难、咳血、过度流泪、嘎嘎作响和结膜炎。尽管接种了许可证和常用疫苗,但这种疾病影响了不同的鸡种和年龄组。对喉、气管、肺和气囊进行组织病理学、超微结构、免疫组织化学检查和分子检测。喉和气管腔的大体检查显示不同类型的渗出物,从卡他性到纤维蛋白原性,还检测到肺炎和气囊炎。组织病理学检查显示,喉、气管、肺和气囊有不同的变化,其特征是含有核内包涵体的合胞体细胞悬挂在亲纤维蛋白渗出物中,并在喉、气管和支气管及支气管旁管腔和气囊中出现。气管病变评分系统用于对不同地区病变的严重程度进行分类。气管病变评分显示,6.02%、26.5%和43.3%的鸟类分别表现出轻度、中度和重度变化,24.18%的鸟类表现出非常严重的变化。此外,严重病例与Qalyubia、Fayoum和Sharkia省有关。免疫组化法检测气管和肺合胞细胞、气管上皮旁炎症细胞中的病毒颗粒。透射电子显微镜能够检测病毒颗粒,并证明嗜异细胞可以被感染。靶向胸苷激酶基因和糖蛋白gJ基因中的一个区域的PCR证实了传染性喉气管炎ILT病毒特异性DNA的存在。总之,解剖病理、免疫组织化学、分子和超微结构的结果显示埃及ILTV的严重程度增加。应收集并检查喉、气管、肺和气囊,以帮助诊断。应考虑良好生物安全水平的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Brazilian Journal of Veterinary Pathology
Brazilian Journal of Veterinary Pathology Veterinary-Veterinary (miscellaneous)
CiteScore
0.60
自引率
0.00%
发文量
24
期刊介绍: The Brazilian Journal of Veterinary Pathology is the official electronic periodical of the Brazilian Association of Veterinary Pathology. The purpose of the BJVP is to publish original full papers, short communications, case reports, letters, reviews (by invited experts) and abstracts of scientific meetings. The preferable subjects is natural and experimental pathology. All the articles are submitted to scientific reviewers.
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