Quercetin supplementation attenuates muscle wasting in cancer-associated cachexia in mice

Q3 Medicine

Nutrition and Healthy Aging Pub Date : 2021-01-20 DOI:10.3233/nha-200084

S. Levolger, S. V. D. Engel, G. Ambagtsheer, J. Ijzermans, R. Bruin

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引用次数: 6

Abstract

BACKGROUND: Quercetin is a flavonoid with reported antioxidant, anti-inflammatory and anti-aging effects, and may limit muscle wasting in cancer cachexia. OBJECTIVE: To investigate the effect of quercetin on muscle wasting in the murine C26 cancer-cachexia model and assess the feasibility of non-invasive micro-CT analysis of skeletal muscle. MATERIALS AND METHODS: Custom CRM(P) diets supplemented with 250 mg/kg quercetin (Q) were obtained. Thirty CD2F1 mice were equally randomized to non-tumor-bearing (NTB), C26 tumor-bearing (TB), TB + Q. All groups started their allocated diet and underwent hindlimb micro-CT. Bodyweight, food intake, and grip-strength were recorded periodically. After 21 days, repeat micro-CT was performed. Gastrocnemius (GCM) and tibialis anterior (TA) muscles were resected. mRNA expression of MuRF1, Atrogin-1, myogenin, and MyoD was determined. RESULTS: NTB and TB + Q gained 9.4% and 5.3% bodyweight respectively, TB lost 3.9%. Hind limb skeletal muscle volume remained stable for NTB and TB + Q, whereas TB decreased from 242.0 mm3 to 212.8 mm3. Mean GCM muscle weight was 175.2 mg (NTB), 171.3 mg (TB + Q) versus 125.5 mg (TB). A tendency towards decreased expression of atrogin-1 and MuRF1 was observed in TB + Q. CONCLUSION: Dietary quercetin supplementation limits bodyweight loss and muscle wasting in the C26-cancer-associated cachexia model.

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槲皮素补充可减轻小鼠癌症相关恶病质中的肌肉萎缩

背景:槲皮素是一种类黄酮，据报道具有抗氧化、抗炎和抗衰老作用，并可能限制癌症恶病质中的肌肉萎缩。目的:探讨槲皮素对小鼠C26癌-恶病质模型肌肉萎缩的影响，并评价骨骼肌无创显微ct分析的可行性。材料与方法:配制添加250 mg/kg槲皮素的CRM(P)饲粮。30只CD2F1小鼠平均随机分为非荷瘤(NTB)、C26荷瘤(TB)、TB + Q组。各组开始分配饮食并进行后肢显微ct检查。定期记录体重、食物摄入量和握力。21天后再次行显微ct检查。切除腓肠肌(GCM)和胫前肌(TA)。检测MuRF1、Atrogin-1、myogenin和MyoD的mRNA表达。结果:NTB和TB + Q体重分别增加9.4%和5.3%，TB体重减少3.9%。NTB和TB + Q患者后肢骨骼肌体积保持稳定，而TB患者后肢骨骼肌体积从242.0 mm3下降到212.8 mm3。平均GCM肌肉重量为175.2 mg (NTB)， 171.3 mg (TB + Q)和125.5 mg (TB)。atrogin-1和MuRF1在TB + Q中有表达降低的趋势。结论:在c26 -癌症相关恶病质模型中，膳食补充槲皮素限制了体重减轻和肌肉萎缩。

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来源期刊

Nutrition and Healthy Aging Agricultural and Biological Sciences-Food Science

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： Nutrition and Healthy Aging is an international forum for research on nutrition as a means of promoting healthy aging. It is particularly concerned with the impact of nutritional interventions on the metabolic and molecular mechanisms which modulate aging and age-associated diseases, including both biological responses on the part of the organism itself and its micro biome. Results emanating from both model organisms and clinical trials will be considered. With regards to the latter, the journal will be rigorous in only accepting for publication well controlled, randomized human intervention trials that conform broadly with the current EFSA and US FDA guidelines for nutritional clinical studies. The journal will publish research articles, short communications, critical reviews and conference summaries, whilst open peer commentaries will be welcomed.