Phytochemical Analysis by LC-HRMS and Antibacterial Activity Of the Ethanol Extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe)

Medical Laboratory Technology Journal Pub Date : 2023-06-13 DOI:10.31964/mltj.v9i1.506

Ratih Dewi Dwiyanti, Anny Thuraidah, Nurlailah Nurlailah

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Abstract

Dracontomelon dao (Blanco) Merr. & Rofe is one of the medicinal plants for the community. This study aims to analyze the ethanol extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) using the LC-HRMS Phytochemical Test and determine its antibacterial activity. Leaf Dracontomelon dao (Blanco) Merr. & Rofe originates from Hulu Sungai Utara, South Kalimantan, Indonesia, macerated with 96% ethanol. Antibacterial test by diffusion was carried out using the well method. LC-HRMS (Liquid Chromatography - High-Resolution Mass Spectrometry) test using the Instrument Thermo Scientific™ Dionex™ Ultimate 3000 RSLCnano UHPLC coupled with Thermo Scientific™ Q Exactive™ High-Resolution Mass Spectrometer. The content of chemical compounds from the ethanol extract, namely flavonoids 125.5 ± 0.433 mgEQ/g, alkaloids (%) 33.945 ± 0.781, saponins (%) 33.093 ± 0.755, tannins (mg/ml) 0.069 ± 0.003, the highest content was triterpenoids 669.8 ± 2,000(mg/ml). The best inhibition of E coli growth with the largest inhibition zone was 24 mm at a 500 mg/ml concentration. The active compounds contained in the leaves of Dracontomelon dao (Blanco) Merr. & Rolfe are quercetin-3β-D-glucoside, anacardic acid, D-glucosamine, azelaic acid, choline, astragalin, quercetin, luteolin, syringic acid. The active ingredient of the ethanol extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) has the potential as an antibacterial and anti-inflammatory that can be added to pharmaceutical preparations. Keywords: Dracontomelon dao (Blanco) Merr. & Rofe; Phytochemical Analysis; Liquid Chromatography - High-Resolution Mass Spectrometry

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生光龙乙醇提取物的LC-HRMS植物化学分析及抗菌活性研究。&))【专业化的表扬!

龙血藤（Blanco）Merr.&Rofe是该社区的药用植物之一。本研究旨在对川芎的乙醇提取物进行分析Rofe），并测定其抗菌活性。叶龙血藤（Blanco）Merr.&Rofe来源于印度尼西亚南加里曼丹的Hulu Sungai Utara，用96%的乙醇浸渍。采用井法进行了扩散抗菌试验。使用Thermo Scientific仪器进行LC-HRMS（液相色谱-高分辨率质谱）测试™ Dionex™ Ultimate 3000 RSLCnano UHPLC与Thermo Scientific耦合™ 轨道阱质谱仪™ 高分辨率质谱仪。乙醇提取物中总黄酮含量为125.5±0.433mgEQ/g，生物碱含量为33.945±0.781，皂苷含量为33.093±0.755，单宁含量为0.069±0.003，其中三萜含量最高，为669.8±2000（mg/ml）。在500mg/ml浓度下，对大肠杆菌生长的最佳抑制作用和最大的抑制区为24mm。龙血藤叶片中所含的活性化合物Rolfe是槲皮素-3β-D-葡萄糖苷、anacardic acid、D-葡糖胺、壬二酸、胆碱、黄芪甲素、槲皮素、木犀草素、丁香酸。桑光乙醇提取物的有效成分Rofe）具有抗菌和抗炎的潜力，可以添加到药物制剂中。关键词：龙血藤Rofe；植物化学分析；液相色谱-高分辨率质谱法

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Medical Laboratory Technology Journal

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2 weeks