Photothermal Antibacterial Activity of Gold Nanorods Stabilized by Phospholipid

IF 1.5 4区物理与天体物理 Q2 PHYSICS, MULTIDISCIPLINARY

Brazilian Journal of Physics Pub Date : 2023-02-09 DOI:10.1007/s13538-023-01265-1

Hasan Hamedani, Hasan Kariminezhad, Hossein Amani

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引用次数: 1

Abstract

In this paper, thermal stability of phospholipid-decorated gold nanorods and their photothermal activity against Staphylococcus epidermidis were experimentally studied. For this aim, cetyl trimethyl ammonium bromide (CTAB) stabilized gold nanostructures were first synthesized, and then the chemical bilayers were exchanged with phospholipid (DMPC). The effects of this replacement on the shape and spectral properties of gold nanorods were analyzed by UV–vis and FTIR as well as transmission electron microscopy (TEM). In addition, thermal heating at 95 ± 2 °C caused CTAB-decorated gold nanorods to decompose in less than half an hour, whereas the phospholipid-modified gold nanorods maintained their spectral characteristics for up to 5 h. Furthermore, while the minimum inhibitory concentration (MIC) for CTAB-AuNRs was obtained equal to 5 μg/ml, DMPC-AuNRs did not indicate significant toxicity in concentrations below 20 μg/ml. The photothermal-induced bactericidal activity of DMPC-gold nanorods was investigated through illumination by a laser with the wavelength of 808 nm. It was demonstrated that treating Staphylococcus epidermidis with the nontoxic concentration of phospholipid-stabilized gold nanorod suspension followed by the laser beam exposure for 15 min resulted in a 4.6 log reduction in the bacterial viable count.

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磷脂稳定金纳米棒光热抗菌活性研究

摘要本文研究了磷脂修饰金纳米棒的热稳定性及其对表皮葡萄球菌的光热活性。为此，首先合成了十六烷基三甲基溴化铵(CTAB)稳定的金纳米结构，然后将其化学双层与磷脂(DMPC)交换。利用紫外可见光谱(UV-vis)和红外光谱(FTIR)以及透射电子显微镜(TEM)分析了这种替代对金纳米棒形状和光谱特性的影响。此外，在95±2℃的温度下加热，ctab修饰的金纳米棒在不到半小时内就会分解，而磷脂修饰的金纳米棒在长达5小时的时间内保持其光谱特征。此外，CTAB-AuNRs的最低抑制浓度(MIC)为5 μg/ml，而DMPC-AuNRs在低于20 μg/ml的浓度下没有明显的毒性。采用波长为808 nm的激光照射，研究了dmpc -金纳米棒的光热致杀菌活性。结果表明，用无毒浓度的磷脂稳定金纳米棒悬浮液处理表皮葡萄球菌，然后用激光束照射15分钟，可使细菌活菌数减少4.6 log。

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来源期刊

Brazilian Journal of Physics 物理-物理：综合

CiteScore

2.50

自引率

6.20%

发文量

189

审稿时长

6.0 months

期刊介绍： The Brazilian Journal of Physics is a peer-reviewed international journal published by the Brazilian Physical Society (SBF). The journal publishes new and original research results from all areas of physics, obtained in Brazil and from anywhere else in the world. Contents include theoretical, practical and experimental papers as well as high-quality review papers. Submissions should follow the generally accepted structure for journal articles with basic elements: title, abstract, introduction, results, conclusions, and references.