Molecular diversity and phylogenetic analysis of Azerbaijan oaks (Quercus spp.) revealed by RAPD markers

Q3 Agricultural and Biological Sciences
G. Aliyeva, J. Ojaghi, S. Rustamova
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引用次数: 0

Abstract

Although Azerbaijan is a center of natural distribution and diversity of genus Quercus around the world, less published data can be found about PCR-based genetic diversity in prevalent oak accessions. The present study aims at finding molecular variation among the Quercus species using RAPD markers. In this study, genomic fingerprinting in five Azerbaijan oak species (Quercus spp.) was carried out using 10 RAPD markers. The result showed that a total of 113 DNA fragments were amplified, of those, 91 bands were polymorphic. The highest PIC, EMR and MI values was observed in the primers OPD-05, OPB-01 and OPB-03. The genetic similarity among the genotypes examined ranged from 0.333 to 0.818 with an average of 0.498. Species-specific DNA fragments were found in all species examined. Based on the cluster analysis using UPGMA method and Jaccard’s similarity index, all the examined oak species fell into three main groups and principal coordinate analysis (PCoA) supported the clustering result. High genetic diversity was found in the tested genetic germplasm of oak species. The results of this study would be very useful for oak improvement program.
亚塞拜然栎(Quercus spp.)分子多样性及系统发育分析
虽然阿塞拜疆是世界上栎属自然分布和多样性的中心,但较少发表的数据可以在流行的橡树资源中找到基于pcr的遗传多样性。本研究旨在利用RAPD标记发现栎属植物间的分子变异。本研究利用10个RAPD标记对5种阿塞拜疆栎(Quercus spp.)进行了基因组指纹图谱分析。结果表明,共扩增出113条DNA片段,其中91条为多态性条带。引物OPD-05、OPB-01和OPB-03的PIC、EMR和MI值最高。各基因型间遗传相似性范围为0.333 ~ 0.818,平均为0.498。在所有被检查的物种中都发现了物种特异性的DNA片段。UPGMA聚类分析和Jaccard相似指数聚类分析表明,各树种可划分为3个主要类群,主坐标分析(PCoA)支持聚类结果。所测栎种遗传资源具有较高的遗传多样性。研究结果对柞木的改良具有一定的指导意义。
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来源期刊
caspian journal of environmental sciences
caspian journal of environmental sciences Environmental Science-Environmental Science (all)
CiteScore
2.30
自引率
0.00%
发文量
0
审稿时长
5 weeks
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