Complete Genome Sequence of Serratia Phage 4S Isolated from Wastewater in Ukraine

Q4 Biochemistry, Genetics and Molecular Biology

Biopolymers and Cell Pub Date : 2021-08-31 DOI:10.7124/bc.000a5d

E. Dukhno, N. Kornienko, S. R. Shybanov, I. Budzanivska, A. Kharina

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引用次数: 1

Abstract

Aim. To isolate and characterize phage of Serratia marcescens bacteria. Methods. Phylogenetic analysis. Results. The complete genome of Serratia phage 4S represents a 173,061-bp double-stranded DNA (dsDNA) with a GC content of 39.9 %. The Basic Local Alignment Search Tool (BLAST) results indicated that the closest relative to Serratia phage 4S is Serratia phage CBH8 (7 % query coverage, 76 % identity). According to the electron micrograph images Serratia phage 4S belongs to the order Caudovirales and the family Myoviridae . Following a phylogenetic analysis of Serratia phage 4S MCP (Major Capsid Protein), our results showed that its MCP was highly homologous to Acinetobacter and Enterobacter phages and on the contrary distant from the MCP of Klebsiella phages. The phylogenetic analysis of Serratia phage 4S DNA helicase indicated that it was highly homologous to Yersinia and Enterobacter phages, and on the contrary distant from DNA helicase of Klebsiella phages. Conclusions. Serratia phage 4S has a lytic pathway, which means that it can be considered for further investigation as a control agent against bacterial infections caused by Serratia marcescens .

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乌克兰污水中分离的沙雷氏菌噬菌体4S全基因组序列

的目标。目的分离粘质沙雷氏菌噬菌体并对其进行鉴定。方法。系统发育分析。结果。Serratia噬菌体4S全长173,061 bp，双链DNA (dsDNA)， GC含量为39.9%。BLAST结果显示，与噬菌体4S最接近的是噬菌体CBH8(查询覆盖率为7%，同源性为76%)。电镜图像显示，4S噬菌体属尾状病毒目肌病毒科。通过对Serratia噬菌体4S MCP (Major Capsid Protein)的系统发育分析，我们发现其MCP与不动杆菌和肠杆菌噬菌体高度同源，而与克雷伯氏菌噬菌体MCP相对较远。系统发育分析表明，Serratia噬菌体4S DNA解旋酶与Yersinia和Enterobacter噬菌体高度同源，而与Klebsiella噬菌体DNA解旋酶距离较远。结论。沙雷氏菌噬菌体4S具有裂解途径，可以考虑作为粘质沙雷氏菌引起的细菌感染的防治剂进行进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biopolymers and Cell Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： “Biopolymer and cell” is published since 1985 at the Institute of Molecular Biology and Genetics NAS of Ukraine under the supervision of the National Academy of Sciences of Ukraine. Our journal covers a wide scope of problems related to molecular biology and genetics including structural and functional genomics, transcriptomics, proteomics, bioinformatics, biomedicine, molecular enzymology, molecular virology and immunology, theoretical bases of biotechnology, physics and physical chemistry of proteins and nucleic acids and bioorganic chemistry.