Ellagic acid inhibits proinflammatory intermediary manufacture by suppressing NF-κB/Akt, VEGF and activating Nrf-2/Caspase-3 signaling pathways in rat testicular damage: a new way for testicular damage cure and in silico approach

IF 2.8 4区医学 Q2 TOXICOLOGY

Toxicology Mechanisms and Methods Pub Date : 2022-03-02 DOI:10.1080/15376516.2022.2046668

A. Aslan, Ozlem Gok, S. Beyaz, Harun Uslu, F. Erman, O. Erman, S. Baspinar

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引用次数: 5

Abstract

Abstract Ellagic acid (EA) has protective effect on testicular damage and this natural compound decreases oxidative damage. The present study aims to examine the preventive effect of ellagic acid (EA) against carbon tetrachloride (CCl4)-induced testicular tissue damage in rats. In testicular tissue, tumor necrosis factor-α (TNF-α), Nuclear factor erythroid-2 related factor 2 (Nrf-2), B-cell lymphoma-2 (Bcl-2), vascular endothelial growth factor (VEGF), Nuclear factor-kappa B (NF-κB), cysteine aspartic proteases (caspase-3) and protein kinase B (Akt) synthesis levels were analyzed by western blot method, reactive oxygen species (ROS) was measured by malondialdehyde (MDA) levels, Glutathione (GSH) level and catalase (CAT) by spectrophotometer. As a result, in comparison with the CCl4 group, caspase-3 and Nrf-2 protein synthesis levels increased in EA + CCl4 group, however, VEGF, Bcl-2, NF-κB, TNF-α and Akt protein synthesis levels decreased, EA application raised GSH levels and CAT activity, reduced MDA levels. In this study, in silico tools were applied to confirm the activity of EA against the cancer with macromolecules such as the above mentioned transcription factors. EA, turned out to show significant activity similarly to some cocrystal ligands, particularly against cancer. These results points out that EA can be used as a testicular damage cure drug in future.

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Ellagic acid通过抑制大鼠睾丸损伤中的NF-κB/Akt、VEGF和激活Nrf-2/Caspase-3信号通路来抑制促炎介质的产生：一种治疗睾丸损伤的新方法和计算机方法

Ellagic acid（EA）对睾丸损伤具有保护作用，这种天然化合物可以减少氧化损伤。本研究旨在观察鞣花酸（EA）对四氯化碳（CCl4）诱导的大鼠睾丸组织损伤的预防作用。睾丸组织中肿瘤坏死因子-α（TNF-α）、核因子-红细胞2型相关因子2（Nrf-2）、B细胞淋巴瘤2（Bcl-2）、血管内皮生长因子（VEGF）、核转录因子-κB（NF-κB）、半胱氨酸天冬氨酸蛋白酶（胱天蛋白酶-3）和蛋白激酶B（Akt）的合成水平采用蛋白质印迹法进行分析，用分光光度计测定丙二醛（MDA）、谷胱甘肽（GSH）和过氧化氢酶（CAT）含量。结果，与CCl4组相比，EA中的胱天蛋白酶-3和Nrf-2蛋白合成水平增加 + CCl4组VEGF、Bcl-2、NF-κB、TNF-α和Akt蛋白合成水平下降，电针提高GSH水平和CAT活性，降低MDA水平。在这项研究中，应用计算机工具来证实EA与大分子如上述转录因子对癌症的活性。EA显示出类似于一些共晶配体的显著活性，特别是对癌症的活性。这些结果表明，电针可以作为一种治疗睾丸损伤的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.