Determination of acute toxicity of sodium pyrithione and its exposure effects on antioxidant enzymes activity, immune status, and histopathological changes in common carp

IF 1.3 4区环境科学与生态学 Q3 ECOLOGY

Chemistry and Ecology Pub Date : 2023-04-21 DOI:10.1080/02757540.2023.2198509

K. Erdoğan

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引用次数: 2

Abstract

ABSTRACT This study determined the LC50-24 h value of sodium pyrithione (NaPT) on Cyprinus carpio at 102.7643 µg/L. Then, 60 healthy fish were randomly allocated into 6 exposure groups, each receiving varying levels of NaPT for different durations (control goup = 0% µg/L NaPT for 24 and 96 h; NaPT-10 = 10% of the LC50-24 h dose of NaPT for 24 and 96 h; NaPT-20 = 20% of the LC50-24 h dose of NaPT for 24 and 96 h). As a result, the increase in malondialdehyde (MDA) levels and the decrease in catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activities in the gill and liver tissue in both duration and dose groups were found to be statistically significant. In addition, NaPT increased serum tumour necrosis factor (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6) levels in both dose groups for both durations. The results revealed that NaPT exposure caused shortening of gill tissue lamellae and vacuolisation (separation) of the secondary lamellar epithelium. Likewise, NaPT exposure was responsible for vacuolisation and hepatocellular degeneration in liver tissue in hepatocytes. In conclusion, the toxicity study, antioxidant enzyme activities, cytokine immune response, and histopathological results indicate that NaPT has a toxic effect on the carp.

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吡硫肽钠的急性毒性测定及其对鲤鱼抗氧化酶活性、免疫状态和组织病理学变化的影响

摘要本研究测定了102.7643µg/L嘧硫酮钠(NaPT)对鲤鱼(Cyprinus carpio)的LC50-24 h值。然后，将60条健康鱼随机分为6个暴露组，每个组接受不同水平的NaPT，持续时间不同(对照组= 0%µg/L NaPT，持续24和96 h;NaPT-10 = 10% LC50-24 h剂量的NaPT持续24和96 h;NaPT-20 = LC50-24 h剂量的20%，持续24和96 h)。结果发现，在持续时间组和剂量组中，鳃和肝脏组织丙二醛(MDA)水平升高，过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性降低，均有统计学意义。此外，NaPT在两个剂量组中均升高血清肿瘤坏死因子(TNF-α)、白细胞介素-1β (IL-1β)、白细胞介素-6 (IL-6)水平。结果表明，NaPT暴露导致鳃组织片层缩短和继发片层上皮空泡化(分离)。同样，NaPT暴露是导致肝组织和肝细胞空泡化和肝细胞变性的原因。综上所述，毒性研究、抗氧化酶活性、细胞因子免疫反应和组织病理学结果表明，NaPT对鲤鱼具有毒性作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry and Ecology 环境科学-环境科学

CiteScore

4.20

自引率

16.00%

发文量

审稿时长

3.7 months

期刊介绍： Chemistry and Ecology publishes original articles, short notes and occasional reviews on the relationship between chemistry and ecological processes. This journal reflects how chemical form and state, as well as other basic properties, are critical in their influence on biological systems and that understanding of the routes and dynamics of the transfer of materials through atmospheric, terrestrial and aquatic systems, and the associated effects, calls for an integrated treatment. Chemistry and Ecology will help promote the ecological assessment of a changing chemical environment and in the development of a better understanding of ecological functions.