Anti-proliferative effects of Cuminum cyminum extraction by co-administration of layered double hydroxide (LDH) nanosheets on SW480 colorectal cancer cell line through apoptosis induction

IF 0.4 Q4 ONCOLOGY

Current Cancer Therapy Reviews Pub Date : 2022-07-07 DOI:10.2174/1573394718666220707112238

Laaya Rostami, H. Soltanzadeh, Farzad Arjomandi Rad

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引用次数: 0

Abstract

Colorectal cancer (CC) is a common cancer in human and one of the major cause of cancer death worldwide. Recently, several therapeutic methods of CC and other malignancies have been developed, but drugs resistance is an important problem in treatment cancer treatment. Therefore, identify and provide novel chemotherapeutic agents is important for treatment of malignancies. In the present study we investigated the anti-cancer activity of Cuminum cyminum extraction in combination of layered double hydroxide (LDH) nanosheets on SW480 CC cell line. The anti-cancer activity of C. cyminum extraction and LDH nanosheets were investigated on SW480 CC cell line by MTT assay. The mRNA expression of apoptosis-related genes (BAX and BCL2) were investigated by Real-Time PCR method. Our results revealed that extraction of C. cyminum significantly decreased proliferation and viability of SW480 CC cell line as a concentration‐time dependent manner. However, anti-proliferation effects of C. cyminum extraction by co-administration of LDH nanosheets was significantly more than its monotherapy. Moreover, expression of BAX and BCL2 genes in the treated SW480 cells was significantly upregulated and downregulated, respectively. In general, present study revealed a significant anti-cancer effects of C. cyminum extraction and LDH nanosheets combination on SW480 CC cells, which may be due to apoptosis induction.

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层状双氢氧化物(LDH)纳米片联合提取Cuminum cyminum通过诱导凋亡对SW480结直肠癌细胞的增殖作用

癌症是人类常见的癌症，也是全球癌症死亡的主要原因之一。近年来，CC和其他恶性肿瘤的治疗方法已经发展起来，但耐药性是癌症治疗中的一个重要问题。因此，鉴定和提供新型化疗药物对恶性肿瘤的治疗具有重要意义。在本研究中，我们研究了菊芋提取物与层状双氢氧化物（LDH）纳米片相结合对SW480CC细胞系的抗癌活性。采用MTT比色法研究了C.cyminum提取物和LDH纳米片对SW480CC细胞株的抗癌活性。采用实时PCR方法检测细胞凋亡相关基因BAX和BCL2的mRNA表达。我们的结果表明，C.cyminum的提取以浓度-时间依赖的方式显著降低了SW480 CC细胞系的增殖和活力。然而，通过共给药LDH纳米片提取C.cyminum的抗增殖作用明显大于其单一疗法。此外，BAX和BCL2基因在处理的SW480细胞中的表达分别显著上调和下调。总的来说，本研究揭示了C.cyminum提取物和LDH纳米片组合对SW480 CC细胞的显著抗癌作用，这可能是由于细胞凋亡诱导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Cancer Therapy Reviews ONCOLOGY-

CiteScore

1.00

自引率

0.00%

发文量

期刊介绍： Current Cancer Therapy Reviews publishes frontier reviews on all the latest advances in clinical oncology, cancer therapy and pharmacology. The journal"s aim is to publish the highest quality review articles dedicated to clinical research in the field. The journal is essential reading for all researchers and clinicians in cancer therapy.