Effects of culture medium composition on the in vitro growth of stem cuttings of Kirengeshoma koreana Nakai, a rare species in Korea

Q3 Social Sciences
Jung Won Shin, Yongxue Chi, Jung Min Kim, Ji Won Lee, Jung Hyun Chae, Cho Hyun Yang, Da Seul Baek, Sang Geul Baek, Hyun Won Yoon, Ha Na Lee, J. Seo, Yeoung Ryul Kim, J. Nam, C. Ahn
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Abstract

Background and objective: Kirengeshoma koreana Nakai is an endemic and endangered species in South Korea. We conducted in vitro propagation and regeneration of K. koreana from stem cuttings to investigate the effects of nine different basal culture media and five different carbohydrate sources on its growth.Methods: Apical segments (at least 1 cm long) collected randomly from a six-week-old K. koreana plantlet grown in vitro were used as explants. In the first experiment, the explants were transferred into square vessels containing 50 mL of nine different basal culture media supplemented with 30 g⋅L-1 sucrose and 3 g⋅L-1 Phytagel. In the second experiment, the explants were transferred into square vessels containing 50 mL half-strength SH medium supplemented with five different carbohydrate sources at 30 g⋅L-1. Each medium was solidified with 3 g⋅L-1 Phytagel. All experiments contained 4 cultures, and the experiments were repeated four times to enhance reproducibility. Data on stem length, shoot fresh weight, leaf width, leaf length, root count, and root length were collected at the end of 8 weeks of culture.Results: ANOVA showed that the basal culture medium had a significant effect on K. koreana growth (p < .001). The half-strength SH medium was the best condition for stem length, shoot fresh weight, leaf width and root length (3.76 ± 0.12 cm, 0.60 ± 0.06 g, 1.19 ± 0.05 cm, and 2.83 ± 0.13 cm, respectively). However, the highest percentage increase in root count (13.00 ± 0.90) was found to occur with half-strength WPM. The effect of different carbohydrate sources on K. koreana growth was significantly different (p < .001), with the exception of stem length and leaf width (p = .26 and p = .09, respectively). Maltose was the best condition for shoot fresh weight (0.90 ± 0.09 g). Although there was no significant difference, sucrose was found to be best for leaf width, leaf length and root length (1.34 ± 0.07 cm, 2.34 ± 0.10 cm, and 3.86 ± 0.19 cm, respectively).Conclusion: This in vitro propagation and regeneration system for K. koreana shows promise in terms of scalability and could help greatly with germplasm conservation and restoration efforts for the species.
培养基组成对韩国珍稀植物韩国金龟子茎插条离体生长的影响
背景与目的:Kirengeshoma koreana Nakai是韩国特有的濒危物种。本研究以韩国赤芍为材料,研究了9种不同的基础培养基和5种不同的碳水化合物源对其生长的影响。方法:以离体培养6周的韩半岛蓟植株为外植体,随机抽取1 cm以上的根茎。在第一个实验中,将外植体移入含有9种不同基础培养基50 mL的方形容器中,培养基中添加30 g⋅L-1蔗糖和3 g⋅L-1 Phytagel。在第二个实验中,将外植体移入含有50 mL半强度SH培养基的方形容器中,并添加5种不同碳水化合物源,浓度为30 g⋅L-1。每种培养基用3 g⋅L-1 Phytagel固化。所有实验包含4个培养,实验重复4次,以提高重复性。在培养8周结束时收集茎长、地上部鲜重、叶宽、叶长、根数和根长等数据。结果:方差分析结果表明,基础培养基对韩国红生长有显著影响(p < 0.001)。茎长、地上部鲜重、叶宽和根长以半强SH培养基为最佳条件(分别为3.76±0.12 cm、0.60±0.06 g、1.19±0.05 cm和2.83±0.13 cm)。然而,半强度WPM的根数增加百分比最高(13.00±0.90)。不同碳水化合物来源对高丽草生长的影响差异极显著(p < 0.001),但茎长和叶宽的影响差异极显著(p = 0.26和p = 0.09)。麦芽糖处理对鲜重(0.90±0.09 g)影响最大,但对叶宽、叶长和根长影响最大(分别为1.34±0.07 cm、2.34±0.10 cm和3.86±0.19 cm)。结论:该系统具有良好的可扩展性,可为韩白桦种质资源的保护和恢复提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of People, Plants, and Environment
Journal of People, Plants, and Environment Social Sciences-Urban Studies
CiteScore
1.10
自引率
0.00%
发文量
42
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