Chemical Constituents and Biological Activities of Mitrella Kentii (Blume) Miq. Leaf Oil

Jurnal Sains Kesihatan Malaysia Pub Date : 2021-01-26 DOI:10.17576/JSKM-2021-1901-17

M. Jasamai, J. Jalil, S. Saadawi, I. Jantan

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Abstract

Chemical constituents and biological activities of the Mitrella kentii leaf oil were investigated in this study. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were used to determine the chemical constituents of the oil. The oil was evaluated for its ability to inhibit prostaglandin E 2 (PGE 2 ) and thromboxane B 2 (TXB 2 ) productions in human whole blood using a radioimmunoassay technique. Its inhibitory effect on platelet-activating factor (PAF) receptor binding with rabbit platelets using 3 H-PAF as a ligand and its free radical scavenging effect on DPPH were also investigated. Caryophyllene oxide (33.8%), E , Z -farnesol (6.9%), benzyl benzoate (6.5%) and viridiflorol (6.5%) were among the major components of the oil. Even though weak inhibitory activities were observed in both PGE 2 and TXB 2 assays, significant results were obtained in both PAF receptor binding inhibition and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging effect with IC 50 value of 6.6 µg/mL and 155.6 µg/mL respectively. These promising activities warrant the development of the oil as an anti-inflammatory agent. DOI : http://dx.doi.org/10.17576/JSKM-2021-1901-17

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Mitrella Kentii (Blume) Miq的化学成分和生物活性。叶油

本文研究了肯蒂叶油的化学成分和生物活性。采用气相色谱法(GC)和气相色谱-质谱法(GC- ms)测定油的化学成分。使用放射免疫测定技术评估了该油抑制人全血中前列腺素e2 (pge2)和血栓素b2 (txb2)产生的能力。研究了其对血小板活化因子(PAF)受体以3h -PAF为配体与兔血小板结合的抑制作用以及对DPPH自由基的清除作用。主要成分为氧化石竹烯(33.8%)、E、Z -法尼醇(6.9%)、苯甲酸苄酯(6.5%)和绿氟醇(6.5%)。虽然在pge2和txb2实验中观察到微弱的抑制活性，但在PAF受体结合抑制和2,2-二苯基-1-苦酰肼(2,2-diphenyl-1-picrylhydrazyl, DPPH)清除作用中获得了显著的结果，ic50值分别为6.6µg/mL和155.6µg/mL。这些有希望的活性保证了油作为抗炎剂的发展。DOI: http://dx.doi.org/10.17576/JSKM-2021-1901-17

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Jurnal Sains Kesihatan Malaysia

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12 weeks