The antifungal effect of propolis extract against cotton wild disease (Verticillium dahliae Kleb.)

Melike Mutlu Yilmaz, Y. Kara, O. Erdoğan
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引用次数: 1

Abstract

The aim of this study is to investigate the antifungal activity of propolis against V. dahliae Kleb. under both in vitro and in vivo conditions. Firstly, the inhibitory effect of the propolis on mycelial growth in Potato Dextrose Agar (PDA) media containing its ethanol extract (PE) at various concentrations (0.003, 0.06, 0.125, 0.25, 0.5, and 1 ppm/mL) was investigated under in vitro conditions. Then to assess the effect of PE on non-defoliating (PHCVd3 isolate) and defoliating (PHCVd47 isolate) pathotypes of V. dahliae, the varieties Giza 45 (resistant), Carmen (tolerant), and Acala SJ2 (susceptible) treated with PEE (1 ppm/mL) were observed in the plant growth chamber up to the 4-6 leaf stage. The whole in vitro experiments were carried out with three replicates, and the studies in vivo experiment were with five replicates depending on a completely randomized parcels design. The most effective dose of PEE with1 ppm/mL dose resulted in 75.2% suppression against the PHCVd3 isolate, while the effect of the same dose against the PHCVd47 isolate was 74.4%. The lowest disease severity index (DSI) values against PHCVd3 and PHCVd47 isolates in cotton cultivars treated with PE were 1.34 and 1.64 in the Giza 45, respectively, and the highest DSI values were 3.80 and 3.90 in the Acala SJ2 cultivar in vivo experiment, respectively. The findings indicate that PE treatment has a promising effect against cotton wilt disease that could be combined with known plant protection strategies.
蜂胶提取物对棉花野生病的抗真菌作用
本研究旨在探讨蜂胶对大丽花弧菌的抑菌活性。在体内和体外条件下。首先,在不同浓度(0.003、0.06、0.125、0.25、0.5和1 ppm/mL)的马铃薯葡萄糖琼脂(PDA)培养基中考察蜂胶对菌丝生长的抑制作用。然后,为了评估PE对大丽花非落叶型(PHCVd3分离株)和落叶型(PHCVd47分离株)的影响,在植物生长室内观察了PEE (1 ppm/mL)处理后的吉萨45(抗性)、卡门(抗性)和Acala SJ2(敏感),直至4-6叶期。整个体外实验采用3个重复,体内实验采用5个重复,采用完全随机包块设计。1 ppm/mL的PEE对PHCVd3的抑制率为75.2%,对PHCVd47的抑制率为74.4%。PE处理棉花品种对PHCVd3和PHCVd47的最低病重指数(DSI)在吉萨45中分别为1.34和1.64,在Acala SJ2品种体内试验中最高病重指数分别为3.80和3.90。研究结果表明,PE处理对棉花枯萎病具有良好的防治效果,可以与已知的植物保护策略相结合。
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来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
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