Oxyresveratrol modulates the immune response in vitro

IF 0.5 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pteridines Pub Date : 2021-01-01 DOI:10.1515/pteridines-2020-0029

S. S. Palabiyik-Yucelik, Simone Moser, K. Becker, Z. Halıcı, Y. Bayir, Marlies Stonig, H. Schennach, D. Fuchs, J. Gostner, K. Kurz

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引用次数: 1

Abstract

Abstract The naturally occurring stilbenoid oxyresveratrol was shown to influence inflammatory and metabolic processes. During cellular immune activation, tryptophan breakdown and neopterin formation via the enzymes indoleamine 2,3-dioxygenase-1 (IDO-1) and GTP-cyclohydrolase, respectively, are induced. Neopterin and the kynurenine to tryptophan ratio are reliable and pertinent biomarkers of Th1-type immune response and are also used in vitro to monitor effects of active plant ingredients on peripheral blood mononuclear cells (PBMCs). We investigated the effects of oxyresveratrol on the activity of the above-mentioned pathways in mitogen-stimulated human PBMC and in the myelomonocytic cell line THP-1. Oxyresveratrol exerted suppressive effects on tryptophan breakdown in both stimulated cell models. Of note, in PBMC, tryptophan breakdown was induced at lower concentrations (5–20 µM) and suppressed at higher treatment concentrations only. Neopterin formation was decreased dose-dependently in stimulated PBMC. In unstimulated PBMC similar, albeit lesser effects were observed. Data indicate that oxyresveratrol exerts distinct and concentration-dependent effects on different immune cell types. IDO-1 is targeted by oxyresveratrol and its activity can be modulated in both directions. Detailed investigations of the interactions would be interesting to fully explore the activity of this phytocompound.

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白藜芦醇对体外免疫反应的调节作用

摘要天然存在的己烯类羟基白藜芦醇对炎症和代谢过程有影响。在细胞免疫激活过程中，分别通过吲哚胺2，3-双加氧酶-1（IDO-1）和GTP环水解酶诱导色氨酸分解和新蝶呤形成。新蝶呤和犬尿氨酸与色氨酸的比例是Th1型免疫反应的可靠和相关的生物标志物，也可在体外用于监测活性植物成分对外周血单核细胞（PBMC）的影响。我们研究了氧白藜芦醇对有丝分裂原刺激的人PBMC和骨髓单核细胞系THP-1中上述途径活性的影响。在两种刺激的细胞模型中，氧白藜芦醇对色氨酸的分解都有抑制作用。值得注意的是，在PBMC中，色氨酸在较低浓度（5-20 µM），并且仅在更高的处理浓度下被抑制。在受刺激的PBMC中，新蝶呤的形成呈剂量依赖性降低。在未刺激的PBMC中观察到类似的，尽管效果较小。数据表明，羟基白藜芦醇对不同类型的免疫细胞具有不同的浓度依赖性作用。IDO-1被羟基白藜芦醇靶向，其活性可以双向调节。对相互作用的详细研究将有助于充分探索这种植物化合物的活性。

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来源期刊

Pteridines 生物-生化与分子生物学

CiteScore

1.20

自引率

25.00%

发文量

审稿时长

>12 weeks

期刊介绍： Pteridines is an open acess international quarterly journal dealing with all aspects of pteridine research. Pteridines are heterocyclic fused ring compounds involved in a wide range of biological functions from the color on butterfly wings to cofactors in enzyme catalysis to essential vitamins. Of the pteridines, 5,6,7,8-tetrahydrobiopterin is the necessary cofactor of several aromatic amino acid monoxygenases, the nitric oxide synthases and glyceryl ether monoxygenase (GEMO). Neopterin plays an essential role in the immune system and is an important biomarker in laboratory medicine for diseases such as HIV, cardiovascular disease, malignant tumors, among others. Topics: -Neopterin, dihydroneopterin, monapterin- Biopterin, tetrahydrobiopterin- Folates, antifolates, riboflavin- Phenylalanine, tyrosine, phenylketonuria, serotonin, adrenalin, noradrenalin, L-DOPA, dopamine, related biogenic amines- Phenylalanine hydroxylase, tyrosine hydroxylase, tryptophan hydroxylase, nitric oxide synthases (iNOS), alkylglycerol monooxygenase (AGMO), dihydropterin reductase, sepiapterin reductase- Homocysteine, mediators of inflammation, redox systems, iron.