Analysis of viruses infecting Cape gooseberry (Physalis peruviana) in southwestern Antioquia (Colombia) suggests a new member of the genus Trichovirus

IF 1 Q3 PLANT SCIENCES

Archives of Phytopathology and Plant Protection Pub Date : 2023-05-09 DOI:10.1080/03235408.2023.2216342

Michelle Bacca, Mónica Higuita, Andrea Restrepo, Yuliana Gallo, M. Marín, P. Gutiérrez

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引用次数: 1

Abstract

Abstract Cape gooseberry (Physalis peruviana) is one of the most important crops in Antioquia (Colombia). However, little is known about the viruses circulating in Cape gooseberry in the southwestern region of the province. In this work, the prevalence of seven viruses (PVY, PVS, PVX, TaLMV, PhyVNV, CGIV-1, and PMTV) was tested by RT-qPCR on seed samples, symptomatic and asymptomatic plants from commercial fields from this region, and virus genomes were investigated using high-throughput sequencing (HTS). The results showed the detection of all seven viruses in at least one sample. PMTV was the most prevalent virus as it was found in six seed samples, eight asymptomatic, and ten symptomatic samples. HTS analysis revealed the presence of a new virus species closely related to the genus Trichovirus and tentatively named as Cape gooseberry associated trichovirus (CgaTV). Near full-length genome sequences were obtained for P. peruviana infecting isolates of TaLMV, PhyVNV, and PVY.

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对感染哥伦比亚安蒂奥基亚西南部醋栗（Physalis peruviana）的病毒的分析表明，毛病毒属中有一个新成员

摘要开普醋栗（Physalis peruviana）是安蒂奥基亚（哥伦比亚）最重要的作物之一。然而，人们对在该省西南部地区的醋栗角传播的病毒知之甚少。在这项工作中，通过RT-qPCR检测了七种病毒（PVY、PVS、PVX、TaLMV、PhyVNV、CGIV-1和PMTV）在该地区商业田地的种子样本、有症状和无症状植物上的流行率，并使用高通量测序（HTS）研究了病毒基因组。结果显示，至少在一个样本中检测到了所有七种病毒。PMTV是最流行的病毒，因为它在6个种子样本、8个无症状样本和10个有症状样本中被发现。HTS分析显示，存在一种与毛病毒属密切相关的新病毒，暂定名为开普醋栗相关毛病毒（CgaTV）。获得了秘鲁P.peruviana感染TaLMV、PhyVNV和PVY分离株的近全长基因组序列。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Phytopathology and Plant Protection Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.20

自引率

0.00%

发文量

100

期刊介绍： Archives of Phytopathology and Plant Protection publishes original papers and reviews covering all scientific aspects of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology, herbal studies and applied nematology and entomology as well as strategies and tactics of protecting crop plants and stocks of crop products against diseases. The journal provides a permanent forum for discussion of questions relating to the influence of plant protection measures on soil, water and air quality and on the fauna and flora, as well as to their interdependence in ecosystems of cultivated and neighbouring areas.