Isolation of Antimicrobial Active Compounds Producing Bacillus subtilis Strain from Bat Guano of Kashmir Cave, Pakistan

IF 2.2 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Geomicrobiology Journal Pub Date : 2023-02-09 DOI:10.1080/01490451.2023.2171164

S. U. U. Jamil, Saeed Ullah, S. Zada, Muhammad Rafiq, A. A. Shah, F. Hasan

引用次数: 0

Abstract

Abstract In this study, bacterial isolates form Bat guano of a lime stone cave capable of producing antibiotics are reported. Screening of bacterial strains was carried out from top, middle, and bottom layer of the guano sample. Out of 20 isolated strains, three showed antimicrobial activity against all tested ATTC strain of common bacterial (both gram positive and gram negative) and fungal species. 16s RNA gene sequencing of the strain showing maximum activity revealed the isolated strain as Bacillus subtilis. Optimum production of antimicrobial compounds took place in TSB medium after 72 h of incubation at 30 °C. FTIR of crude extract showed chemical similarity with bacitracin. GCMS analysis of the crude extract revealed a range of antimicrobial and anticancer compounds. It is concluded that Bacillus subtilis strain isolated from Bat Guano is a potential source of novel antimicrobial compounds of broad-spectrum nature and have inhibitory effects against potential pathogens.

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巴基斯坦克什米尔山洞蝙蝠鸟粪中枯草芽孢杆菌抗菌活性物质的分离

摘要在本研究中，从一个石灰石洞穴的蝙蝠粪中分离出能够产生抗生素的细菌。从鸟粪样品的上层、中层和底层进行菌株筛选。在20个分离菌株中，有3个对所有测试的普通细菌（革兰氏阳性和革兰氏阴性）和真菌的ATTC菌株显示出抗菌活性。显示最大活性的菌株的16s RNA基因测序显示分离菌株为枯草芽孢杆菌。72后在TSB培养基中进行了抗菌化合物的最佳生产 30培养h °C。粗提物的FTIR与杆菌肽具有相似的化学性质。粗提取物的GCMS分析揭示了一系列抗微生物和抗癌化合物。从蝙蝠粪中分离的枯草芽孢杆菌菌株是一种潜在的广谱新型抗菌化合物来源，对潜在的病原体具有抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Geomicrobiology Journal 环境科学-地球科学综合

CiteScore

4.80

自引率

8.70%

发文量

审稿时长

3.3 months

期刊介绍： Geomicrobiology Journal is a unified vehicle for research and review articles in geomicrobiology and microbial biogeochemistry. One or two special issues devoted to specific geomicrobiological topics are published each year. General articles deal with microbial transformations of geologically important minerals and elements, including those that occur in marine and freshwater environments, soils, mineral deposits and rock formations, and the environmental biogeochemical impact of these transformations. In this context, the functions of Bacteria and Archaea, yeasts, filamentous fungi, micro-algae, protists, and their viruses as geochemical agents are examined. Articles may stress the nature of specific geologically important microorganisms and their activities, or the environmental and geological consequences of geomicrobiological activity. The Journal covers an array of topics such as: microbial weathering; microbial roles in the formation and degradation of specific minerals; mineralization of organic matter; petroleum microbiology; subsurface microbiology; biofilm form and function, and other interfacial phenomena of geological importance; biogeochemical cycling of elements; isotopic fractionation; paleomicrobiology. Applied topics such as bioleaching microbiology, geomicrobiological prospecting, and groundwater pollution microbiology are addressed. New methods and techniques applied in geomicrobiological studies are also considered.