Resolvin D1 Regulates Porphyromonas Gingivalis Lipopolysaccharide-induced Del-1 and Cytokine Expressions in Human Gingival Fibroblasts

Abstract

Purpose: To detect the effect of Resolvin D1 (RvD1) on Developmental endothelial locus-1 (Del-1) and cytokine expressions of human gingival fibroblast cells exposed to Porphyromonas gingivalis lipopolysaccharide (P. gingivalis-LPS). Material and Methods: The effect of RvD1 on cell viability of human gingival fibroblasts exposed to P. gingivalis-LPS were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Meanwhile, the effect of RvE1 on Del-1 and cytokine (IL-1β, IL-6, IL-8, IL-10, IL-17) expressions of human gingival fibroblasts exposed to P. gingivalis-LPS were studied by real-time PCR experiment, statistical analysis was performed using GraphPad Prism version 5 for Windows. Results: Cell viability assay results demonstrated that RvD1 concentrations upregulated cell number compared to control group at 24 and at 72 hours. While RvD1 reduced IL-6, IL-8, IL-17 mRNA expression, IL-10 and Del-1 mRNA expression increased as a time- and dose-dependent manner. Also, IL-1β was not affected by RvD1 treatments. Conclusion: The increased expression of Del-1 and IL-10 by RvD1 down-regulated the pro-inflammatory cytokine expressions induced by P. gingivalis-LPS in gingival fibroblast. RvD1 displayed regulatory effects on gingival inflammation in P. gingivalis LPS-induced cell culture experiment. In particular, results of study display that Del-1 induced by RvD1 may have therapeutic potential to modulate periodontal inflammation.