Tuber Development and Propagation Are Inhibited by GA3 on DELLA-dependent Pathway in Cyperus rotundus

IF 2.1 2区农林科学 Q2 AGRONOMY

Weed Science Pub Date : 2023-09-04 DOI:10.1017/wsc.2023.47

Chengcai Wei, Dan-Li Fan, Shu-yu Liu, Shan-Chi Yi, Shi-Xian Yu, Guo-Chao Zhao, Xiao-Liang Liu, W. Tang

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Abstract

Cyperus rotundus L. is a globally distributed noxious weed that poses a significant challenge for control due to its fast and efficient propagation through the tuber, which is the primary reproductive organ. Gibberellic acid (GA3) has proved to be crucial for tuberization in tuberous plants. Therefore, understanding the relationship between GA3 and tuber development and propagation of C.roundus will provide valuable information for controlling this weed. This study shows that the content of GA3 decreases with tuber development, which corresponds to lower expression of bioactive GA3 synthesis genes (CrGA20ox, two CrGA3oxs) and two up-regulated GA3 catabolism genes (CrGA2oxs), indicating that GA3 is involved in tuber development. Simultaneously, the expressions of CrDELLAs and CrGID1 decline with tuber growth and GA3 decreasing, and Yeast two-hybrid (Y2H) assays confirm that the GA3 signaling is DELLA-dependent. Furthermore, exogenous application of GA3 markedly reduces the number and the width of tuber, and represses the growth of tuber chain, further confirming the negative impact that GA3 has on tuber development and propagation. Taken together, these results demonstrate that GA3 is involved in tuber development and regulated by the DELLA-dependent pathway in C. rotundus, and plays a negative role in tuber development and propagation.

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GA3通过DELLA依赖途径抑制圆柏块茎发育和繁殖

圆柏是一种分布在全球的有毒杂草，由于其通过块茎（主要生殖器官）快速高效繁殖，对控制构成了重大挑战。赤霉素（GA3）已被证明对块茎植物的块茎形成至关重要。因此，了解GA3与圆线虫块茎发育和繁殖的关系，将为控制该杂草提供有价值的信息。本研究表明，GA3含量随着块茎发育而降低，这与生物活性GA3合成基因（CrGA20ox，两个CrGA3oxs）和两个上调的GA3分解代谢基因（CrGA2oxs）的表达降低相对应，表明GA3参与了块茎发育。同时，CrDELLA和CrGID1的表达随着块茎生长和GA3的减少而下降，酵母双杂交（Y2H）测定证实GA3信号传导是DELLA依赖性的。此外，外源施用GA3显著减少了块茎的数量和宽度，抑制了块茎链的生长，进一步证实了GA3对块茎发育和繁殖的负面影响。总之，这些结果表明，GA3参与圆白菜块茎的发育，并受DELLA依赖性途径的调节，在块茎的发育和繁殖中起着负面作用。

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来源期刊

Weed Science 农林科学-农艺学

CiteScore

4.60

自引率

12.00%

发文量

审稿时长

12-24 weeks

期刊介绍： Weed Science publishes original research and scholarship in the form of peer-reviewed articles focused on fundamental research directly related to all aspects of weed science in agricultural systems. Topics for Weed Science include: - the biology and ecology of weeds in agricultural, forestry, aquatic, turf, recreational, rights-of-way and other settings, genetics of weeds - herbicide resistance, chemistry, biochemistry, physiology and molecular action of herbicides and plant growth regulators used to manage undesirable vegetation - ecology of cropping and other agricultural systems as they relate to weed management - biological and ecological aspects of weed control tools including biological agents, and herbicide resistant crops - effect of weed management on soil, air and water.