Application of the LUminometric Methylatoion Assay for plant ecological researches: the study of global DNA methylation in leaves of Elodea canadensis under laboratory conditions and in leaves of fen orchid from wild populations

Q3 Agricultural and Biological Sciences

Plant Omics Pub Date : 2020-05-20 DOI:10.21475/poj.13.01.20.p2111

N. Škute, Marina Savicka, A. Petjukevics, N. Harlamova

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引用次数: 1

Abstract

The epigenetic changes in the genome of plants are one of the important regulatory mechanisms in response to the environmental factors. The LUminometric Methylation Assay (LUMA) requires a relatively small DNA amount, a short processing time and is easily adapted for species with a non-resolved genome. The LUMA has not been previously used for ecological research of plants. In this research, LUMA was used for the first time to investigate the changes of global DNA methylation under different environmental factors in the leaves of different plants. The influence of salinity on global DNA methylation was studied on aquatic macrophyte Elodea canadensis Michx, which grew in aquatic tanks under different NaCl concentrations. After the third week of growth, the HpaII/MspI ratio was measured by LUMA and global DNA methylation percentages were calculated. The results showed salt stressinduced changes in the global DNA methylation level in E.canadensis leaves, compared to control. The response was salt dosedependent. The changes of global DNA methylation in wildlife plant populations were analogically assessed on fen orchid Liparis loeselii (L.) Rich. It was shown that global DNA methylation level was higher in leaves of these plants in Engure Lake, where there are temporary changes in water regime, compared to leaves of plants from other places. It was assumed that global GC-DNA methylation plays an essential role in the survival of this plant. Therefore, we show the possibilities of using the LUMA method for epigenetic study of different plants ecological researches.

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荧光甲基化法在植物生态学研究中的应用:实验室条件下加拿大叶香和野生凤兰叶片DNA甲基化的研究

植物基因组的表观遗传学变化是对环境因素作出反应的重要调控机制之一。微量甲基化测定（LUMA）需要相对较小的DNA量、较短的处理时间，并且很容易适用于基因组未解析的物种。LUMA以前没有用于植物的生态学研究。在本研究中，首次使用LUMA研究了不同环境因素对不同植物叶片全局DNA甲基化的影响。研究了盐度对不同NaCl浓度下生长在水槽中的大型水生植物加拿大藻（Elodea canadensis Michx）DNA甲基化的影响。生长第三周后，通过LUMA测量Hpal/MspI比率，并计算总体DNA甲基化百分比。结果表明，与对照相比，盐胁迫诱导加拿大E.canadensis叶片的整体DNA甲基化水平发生变化。反应依赖于盐的剂量。对野生植物种群中DNA甲基化的变化进行了类比评估。研究表明，与其他地方的植物叶片相比，Engure湖的这些植物叶片的整体DNA甲基化水平更高，那里的水情发生了暂时的变化。据推测，全球GC-DNA甲基化在这种植物的生存中起着至关重要的作用。因此，我们展示了利用LUMA方法对不同植物生态研究进行表观遗传学研究的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Omics 生物-植物科学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Plant OMICS is an international, peer-reviewed publication that gathers and disseminates fundamental and applied knowledge in almost all area of molecular plant and animal biology, particularly OMICS-es including: Coverage extends to the most corners of plant and animal biology, including molecular biology, genetics, functional and non-functional molecular breeding and physiology, developmental biology, and new technologies such as vaccines. This journal also covers the combination of many areas of molecular plant and animal biology. Plant Omics is also exteremely interested in molecular aspects of stress biology in plants and animals, including molecular physiology.