Role of HIV-1 envelope protein gp120 in neuronal injury-induced cognitive impairment

Q4 Immunology and Microbiology

中华微生物学和免疫学杂志 Pub Date : 2020-01-31 DOI:10.3760/CMA.J.ISSN.0254-5101.2020.01.006

Y. Wan, Weijun Yang, Z. Gong, Zhijie Zeng, Hanyun Zhang, Ke Lyu

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引用次数: 0

Abstract

Objective To investigate the role of HIV-1 envelope protein gp120 in cognitive impairment induced by neuronal damage. Methods Western blot and immunofluorescence assay were used to detect microglia activation, inflammatory factor expression and neuronal damage after gp120 treatment. Neuronal damage and neurocognitive performance in gp120-transgenic mice were evaluated using immunohistochemical staining and behavioral analysis, respectively. Results In vivo and in vitro experiments showed that HIV-1 gp120 significantly induced the expression of caspase-1 and IL-1β, and indirectly caused neuronal synaptic shortening and neuronal damage (P<0.05). Compared with wild-type mice, gp120-transgenic mice showed significant cortical and hippocampal glial activation, neuronal loss, dendritic damage and neurocognitive disorders. Conclusions HIV-1 gp120 might cause neuronal damage through activating the release of inflammatory factor by microglia and involve in neurocognitive impairment. Key words: HIV-1; gp120; Cognitive impairment; gp120-transgenic mouse; Microglia

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HIV-1包膜蛋白gp120在神经元损伤性认知障碍中的作用

目的探讨HIV-1包膜蛋白gp120在神经元损伤所致认知功能障碍中的作用。方法采用Western blot和免疫荧光法检测gp120治疗后小胶质细胞活化、炎症因子表达及神经元损伤情况。采用免疫组化染色和行为学分析分别评价gp120转基因小鼠的神经损伤和神经认知能力。结果体内和体外实验显示，HIV-1 gp120显著诱导caspase-1和IL-1β的表达，并间接导致神经元突触缩短和神经元损伤(P<0.05)。与野生型小鼠相比，gp120转基因小鼠表现出明显的皮质和海马胶质细胞激活、神经元丢失、树突损伤和神经认知障碍。结论HIV-1 gp120可能通过激活小胶质细胞释放炎症因子导致神经损伤，参与神经认知功能障碍。关键词:HIV-1;gp120;认知障碍;gp120-transgenic鼠标;小神经胶质细胞

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来源期刊

中华微生物学和免疫学杂志 Immunology and Microbiology-Virology

CiteScore

0.50

自引率

0.00%

发文量

6906

期刊介绍： Chinese Journal of Microbiology and Immunology established in 1981. It is one of the series of journal sponsored by Chinese Medical Association. The aim of this journal is to spread and exchange the scientific achievements and practical experience in order to promote the development of medical microbiology and immunology. Its main contents comprise academic thesis, brief reports, reviews, summaries, news of meetings, book reviews and trends of home and abroad in this field. The distinguishing feature of the journal is to give the priority to the reports on the research of basic theory, and take account of the reports on clinical and practical skills.