Genetic diversity and population structure of Dermatophagoides farinae based on microsatellite markers

IF 0.9 3区农林科学 Q3 ENTOMOLOGY

International Journal of Acarology Pub Date : 2022-04-28 DOI:10.1080/01647954.2022.2069284

Mengnan Li, W. Fang, Huihui Wang, Zijian Chen, Xing-Yao Li, Yi Liu, Yongqi Li, Duo Wang, Qingyu Huang, Wenyan Lu, Renrui Han, Liang Hong, E. Sun

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引用次数: 2

Abstract

ABSTRACT Dermatophagoides farinae is one of the most important allergens that cause allergic diseases and trigger serious harm to the human body. D.farinae is widely distributed, and the development of genetic markers and genetic information regarding the populations of this species are needed to establish control strategies. The aim is to develop a microsatellite marker and assess the genetic diversity and population structure of different populations of D. farinae. The genomic DNA of D. farinae was extracted and sequenced by NGS analysis. Microsatellite markers were identified and screened with MISA software. Primers were designed with Primer5.0 and screened by capillary electrophoresis detection. Genotyping was implemented with GeneMarker 2.2. Genetic diversity and population structure were analysed by related softwares. Twelve microsatellite markers detected 66 alleles in 256 individuals of D. farinae from 12 geographic populations. The average allele number (Na), effective allele number (Ne), observed heterozygosity (Ho), and expected heterozygosity (He) were 4.431, 2.610, 1.056 and 0.484, respectively. Genetic variation existed within populations and there was no correlation between geographic distance and genetic distance (Fst) (P = 0.160). Structure analysis showed D. farinae is largely divided into three groups. The genetic diversity of the geographical population of D. farinae is relatively high.

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基于微卫星标记的粉蚧遗传多样性及群体结构研究

摘要粉尘性皮肤病是引起过敏性疾病并对人体造成严重危害的重要过敏原之一。粉蚧分布广泛，需要开发有关该物种种群的遗传标记和遗传信息，以制定控制策略。目的是开发一个微卫星标记，并评估不同群体的遗传多样性和群体结构。提取粉蚧基因组DNA，通过NGS分析进行测序。利用MISA软件对微卫星标记进行了鉴定和筛选。用Primer5.0设计引物，通过毛细管电泳检测进行筛选。用GeneMarker 2.2进行基因分型。利用相关软件对遗传多样性和种群结构进行了分析。12个微卫星标记在来自12个地理群体的256个粉蚧个体中检测到66个等位基因。平均等位基因数目（Na）、有效等位基因数（Ne）、观察到的杂合性（Ho）和预期杂合度（He）分别为4.431、2.610、1.056和0.484。群体间存在遗传变异，地理距离与遗传距离（Fst）无相关性（P=0.160）。粉蚧地理种群的遗传多样性较高。

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来源期刊

International Journal of Acarology 生物-昆虫学

CiteScore

2.20

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The International Journal of Acarology has a global readership and publishes original research and review papers on a wide variety of acarological subjects including: • mite and tick behavior • biochemistry • biology • control • ecology • evolution • morphology • physiology • systematics • taxonomy (single species descriptions are discouraged unless accompanied by additional new information on ecology, biology, systematics, etc.) All submitted manuscripts are subject to initial appraisal by the Editor. If the English is not of a quality suitable for reviewers, the manuscript will be returned. If found suitable for further consideration, it will be submitted to peer review by independent, anonymous expert referees. All peer review is single blind.